GM11535
Somatic cell hybrid
Description:
NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Family Member
|
14
|
|
Confirmation
|
Karyotypic analysis and Southern blot hybridization
|
|
ISCN
|
Human/Chinese hamster somatic cell hybrid retaining human chromosome #14
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, and 2q13, IL1A. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p25-p24.2, D3S1304, 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p23-p22, DEF1, and 8q24,TG. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9pter-p22, D9S178, and 9q22.3-q31, ALDOB. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10p13, VIM. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 14q11.2, NP, and 14q32.1, PI. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, and 16q22.1, LCAT. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q21.3-q23, MPO1, and 17q, GAS. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.3, BCEI. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q12.1-q13.1, D22S300. |
| |
| Remarks |
Line MHR14; produced by the fusion of human leukocytes with Chinese hamster ovary cells; originally selected in medium with sodium chromate; grown in culture medium without selection at 34C; retains human chromosome #14 in 100% of cells |
| Lopes M, Louzada S, Ferreira D, Veríssimo G, Eleutério D, Gama-Carvalho M, Chaves R, Human Satellite 1A analysis provides evidence of pericentromeric transcription BMC biology21:28 2022 |
| PubMed ID: 36755311 |
| |
| Krona C, Ejeskär K, Carén H, Abel F, Sjöberg RM, Martinsson T, A novel 1p362 located gene, APITD1, with tumour-suppressive properties and a putative p53-binding domain, shows low expression in neuroblastoma tumours British journal of cancer91:1119-30 2004 |
| PubMed ID: 15328517 |
| |
| Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B, The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology Proceedings of the National Academy of Sciences of the United States of America101:1731-6 2004 |
| PubMed ID: 14757826 |
| |
| Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ, Identification and characterization of human taste receptor genes belonging to the TAS2R family Cytogenetic and genome research98:45-53 2003 |
| PubMed ID: 12584440 |
| |
| Hansford LM, Smith SA, Haber M, Norris MD, Cheung B, Marshall GM, Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2) Cytogenetic and genome research101:17-23 2003 |
| PubMed ID: 14571131 |
| |
| Janssens B, Mohapatra B, Vatta M, Goossens S, Vanpoucke G, Kools P, Montoye T, van Hengel J, Bowles NE, van Roy F, Towbin JA, Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy Human genetics112:227-36 2003 |
| PubMed ID: 12596047 |
| |
| Quade BJ, Weremowicz S, Neskey DM, Vanni R, Ladd C, Dal Cin P, Morton CC, Fusion transcripts involving HMGA2 are not a common molecular mechanism in
uterine leiomyomata with rearrangements in 12q15. Cancer Res63(6):1351-8 2003 |
| PubMed ID: 12649198 |
| |
| Tarafa G, Prat E, Risques RA, González S, Camps J, Grau M, Guinó E, Moreno V, Esteller M, Herman JG, Germà JR, Miró R, Peinado MA, Capellá G, Common genetic evolutionary pathways in familial adenomatous polyposis tumors Cancer research63:5731-7 2003 |
| PubMed ID: 14522893 |
| |
| Berend SA,Horwitz J,McCaskill C,Shaffer L, Identification of Uniparental Disomy Following Prenatal Detection of Robertsonian Translocations and Isochromosomes. Am J Hum Genet66:1787-1793 2000 |
| PubMed ID: 10775524 |
| |
| Dear PH, Bankier AT, Piper MB, A high-resolution metric HAPPY map of human chromosome 14. Genomics48:232-41 1998 |
| PubMed ID: 9521877 |
| |
| Dyomin VG, Rao PH, Dalla-Favera R, Chaganti RS, BCL8, a novel gene involved in translocations affecting band 15q11-13 in diffuse large-cell lymphoma. Proc Natl Acad Sci U S A94(11):5728-32 1997 |
| PubMed ID: 9159141 |
| |
| Gonzalez IL, Sylvester JE, Beyond ribosomal DNA: on towards the telomere. Chromosoma105:431-7 1997 |
| PubMed ID: 9211970 |
| |
| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
| |
| Sherrington R, Rogaev EI, Liang Y, Rogaeva EA, Levesque G, Ikeda M, Chi H, Lin C, Li G, Holman K, et al, Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease [see comments] Nature375:754-60 1995 |
| PubMed ID: 7596406 |
| |
| Mares A Jr, Ledbetter SA, Ledbetter DH, Roberts R, Hejtmancik JF, Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR. Genomics11:215-8 1991 |
| PubMed ID: 1765380 |
| Split Ratio |
1:5 |
| Temperature |
34 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|