GM23720
iPSC from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Apparently healthy iPSCs Apparently Healthy Collection |
Protocols |
Protocol PDF |
Biopsy Source
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Peripheral vein
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Sample Source
|
iPSC from B-Lymphocyte
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Race
|
White
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Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis and Case history
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ISCN
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46,XX[20].arr[hg19]Xp21.1(31936202_32163760)x1, 16q11.2q24.3(46534976_90163275) hmz
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
|
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Passage Frozen |
51 |
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Induced Pluripotent Stem Cell |
The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and the PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSCs and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
|
Remarks |
Induced pluripotent stem cell line derived from GM02254 Lymphoid. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is WARF. |
Kim MY, Kim MJ, Lee C, Lee J, Kim SS, Hong S, Kim HT, Seo J, Yoon KJ, Han S, Trametinib activates endogenous neurogenesis and recovers neuropathology in a model of Alzheimer's disease Experimental & molecular medicine55:2177-2189 2022 |
PubMed ID: 37779138 |
|
Lee J, Lee H, Lee H, Shin M, Shin MG, Seo J, Lee EJ, Park SA, Park S, ANKS1A regulates LDL receptor-related protein 1 (LRP1)-mediated cerebrovascular clearance in brain endothelial cells Nature communications14:8463 2022 |
PubMed ID: 38123547 |
|
Morris GE, Denniff MJ, Karamanavi E, Andrews SA, Kostogrys RB, Bountziouka V, Ghaderi-Najafabadi M, Shamkhi N, McConnell G, Kaiser MA, Carleton L, Schofield C, Kessler T, Rainbow RD, Samani NJ, Webb TR, The integrin ligand SVEP1 regulates GPCR-mediated vasoconstriction via integrins a9ß1 and a4ß1 British journal of pharmacology179:4958-4973 2022 |
PubMed ID: 35802072 |
|
Bogacheva MS, Bystriakova MA, Lou YR, Thyroid Hormone Effect on the Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-Like Cells Pharmaceuticals (Basel, Switzerland)14:4958-4973 2021 |
PubMed ID: 34200130 |
|
Cho S, Lee H, Jung M, Hong K, Woo SH, Lee YS, Kim BJ, Jeon MY, Seo J, Mun JY, Neuromyelitis optica (NMO)-IgG-driven organelle reorganization in human iPSC-derived astrocytes FASEB journal : official publication of the Federation of American Societies for Experimental Biology35:e21894 2021 |
PubMed ID: 34460995 |
|
Choi HJ, Torizal FG, Shinohara M, Sakai Y, Differentiation of Human Induced Pluripotent Stem Cells into Definitive Endoderm Using Simple Dialysis Culture Device Methods in molecular biology (Clifton, NJ)2454:731-742 2021 |
PubMed ID: 33900573 |
|
Lee SI, Jeong W, Lim H, Cho S, Lee H, Jang Y, Cho J, Bae S, Lin YT, Tsai LH, Moon DW, Seo J, APOE4-carrying human astrocytes oversupply cholesterol to promote neuronal lipid raft expansion and Aß generation Stem cell reports16:2128-2137 2021 |
PubMed ID: 34450034 |
|
Lim ET, Chan Y, Dawes P, Guo X, Erdin S, Tai DJC, Liu S, Reichert JM, Burns MJ, Chan YK, Chiang JJ, Meyer K, Zhang X, Walsh CA, Yankner BA, Raychaudhuri S, Hirschhorn JN, Gusella JF, Talkowski ME, Church GM, Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder Nature communications13:3243 2020 |
PubMed ID: 35688811 |
|
Lim ET, Chan Y, Dawes P, Guo X, Erdin S, Tai DJC, Liu S, Reichert JM, Burns MJ, Chan YK, Chiang JJ, Meyer K, Zhang X, Walsh CA, Yankner BA, Raychaudhuri S, Hirschhorn JN, Gusella JF, Talkowski ME, Church GM, Human retinal ganglion cell neurons generated by synchronous BMP inhibition and transcription factor mediated reprogramming npg regenerative medicine13:3243 2020 |
PubMed ID: 37773257 |
Passage Frozen |
51 |
Split Ratio |
1:15 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
mTeSR1 |
Serum |
none |
Substrate |
Matrigel |
Supplement |
- |
|
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