AG06115
Fibroblast from Skin, Thigh
Description:
CALLICEBUS
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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| Subcollection |
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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50,XY,inv(7)
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Species
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Callicebus moloch
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Common Name
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dusky titi
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Remarks
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| PDL at Freeze |
14 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remark |
Paraguayan titi. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. Age of the donor is estimated. The karyotype is 50,XY,inv(7). |
| Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
| PubMed ID: 18799582 |
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| Steiper ME, Parikh SJ, Zichello JM, Phylogenetic analysis of the promoter region of the CD40L gene in primates and other mammals Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases8:406-13 2006 |
| PubMed ID: 17275421 |
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| Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005 |
| PubMed ID: 15689398 |
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| Dumas F, Bigoni F, Stone G, Sineo L, Stanyon R, Mapping genomic rearrangements in titi monkeys by chromosome flow sorting and multidirectional in-situ hybridization Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology13:85-96 2004 |
| PubMed ID: 15791414 |
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| Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989 |
| PubMed ID: 2805817 |
| Cumulative PDL at Freeze |
14 |
| Passage Frozen |
8 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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