AG08346
Fibroblast from Skin, Arm
Description:
MACACA
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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Subcollection |
Animals |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
42,XY
|
Species
|
Macaca nemestrina
|
Common Name
|
pigtailed macaque
|
Remarks
|
|
PDL at Senescence |
34 |
PDL at Freeze |
2 |
Passage Frozen |
1 |
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Genbank |
COX1 |
Remark |
The skin biopsy was taken post-mortem on 7/25/85 from the inner aspect of the upper arm of a fetus of 130 days gestational age. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 42,XY with 8% of the cells examined showing random chromosome loss. Other cultures from this monkey are AG08347 and AG08351. |
PDL at Senescence |
34 |
Cumulative PDL at Freeze |
2 |
Passage Frozen |
1 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
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