AG08427
Fibroblast from Lung, Lung
Description:
MACACA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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| Subcollection |
Animals |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XY
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Species
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Macaca nemestrina
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Common Name
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pigtailed macaque
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Remarks
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| PDL at Senescence |
14 |
| PDL at Freeze |
3 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remark |
The lung biopsy was taken post-mortem on 9/18/85 from a fetus of 80 days gestational age. The culture was initiated using explants of minced lung tissue. Cell morphology is fibroblast-like. The karyotype is 42,XY; normal diploid male with 2% of the cells examined showing random chromosome loss. A matching skin fibroblast culture from this monkey is AG08426. |
| PDL at Senescence |
14 |
| Cumulative PDL at Freeze |
3 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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