AG08427
Fibroblast from Lung, Lung
Description:
MACACA
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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Subcollection |
Animals |
Biopsy Source
|
Lung
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Lung
|
Transformant
|
Untransformed
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
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ISCN
|
42,XY
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Species
|
Macaca nemestrina
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Common Name
|
pigtailed macaque
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Remarks
|
|
PDL at Senescence |
14 |
PDL at Freeze |
3 |
Passage Frozen |
1 |
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remark |
The lung biopsy was taken post-mortem on 9/18/85 from a fetus of 80 days gestational age. The culture was initiated using explants of minced lung tissue. Cell morphology is fibroblast-like. The karyotype is 42,XY; normal diploid male with 2% of the cells examined showing random chromosome loss. A matching skin fibroblast culture from this monkey is AG08426. |
PDL at Senescence |
14 |
Cumulative PDL at Freeze |
3 |
Passage Frozen |
1 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
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