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AG17930
Adipose stromal cell
from
Skin, Abdomen
Description:
APPARENTLY HEALTHY INDIVIDUAL
Affected:
No
Sex:
Female
Age:
51
YR
(At Sampling)
Sample Description
Overview
Characterizations
Phenotypic Data
Culture Protocols
Repository
NIA Aging Cell Culture Repository
Subcollection
Apparently Healthy Collection
Biopsy Source
Abdomen
Cell Type
Adipose stromal cell
Tissue Type
Fat
Transformant
Untransformed
Relation to Proband
proband
Confirmation
Clinical summary/Case history
Species
Homo
sapiens
Common Name
Human
Remarks
The fat tissue was removed from the subject's abdomen and waist ante-mortem during elective cosmetic tumescent liposuction on 10/16/02. This primary culture was initiated on 10/16/02 by collagenase digestion of fat tissue. Donor subject (5 ft 2 in) weighed 128 lbs (58.2 kgs) at the time of the procedure.This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 59% of the cells observed at passage 5 showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN.
PDL at Senescence
7
PDL at Freeze
4.85
Passage Frozen
2
IDENTIFICATION OF SPECIES OF ORIGIN
Species of Origin confirmed by LINE assay
Remark
The fat tissue was removed from the subject's abdomen and waist ante-mortem during elective cosmetic tumescent liposuction on 10/16/02. This primary culture was initiated on 10/16/02 by collagenase digestion of fat tissue. Donor subject (5 ft 2 in) weighed 128 lbs (58.2 kgs) at the time of the procedure.This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 59% of the cells observed at passage 5 showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN.
No data is available
No data is available
No data is available
PDL at Senescence
7
Cumulative PDL at Freeze
8.31
Passage Frozen
2
Split Ratio
1:20
Temperature
37 C
Percent CO2
5%
Percent O2
AMBIENT
Medium
Adipose Stromal Cell Medium
Serum
0% fetal bovine serum Not inactivated
Substrate
Human Fibronectin
Pricing
Commercial:
$257.00
USD
Academic &
Non-profit:
$103.00
USD
NIA Grantees:
$47.00
USD
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