AG17930

AG17930 Adipose stromal cell from Skin, Abdomen

Description:

APPARENTLY HEALTHY INDIVIDUAL

Affected:

No

Sex:

Female

Age:

51 YR (At Sampling)

Repository

NIA Aging Cell Culture Repository

Subcollection

Apparently Healthy Collection

Biopsy Source

Abdomen

Cell Type

Adipose stromal cell

Tissue Type

Fat

Transformant

Untransformed

Relation to Proband

proband

Confirmation

Clinical summary/Case history

Species

Homo sapiens

Common Name

Human

Remarks

The fat tissue was removed from the subject's abdomen and waist ante-mortem during elective cosmetic tumescent liposuction on 10/16/02. This primary culture was initiated on 10/16/02 by collagenase digestion of fat tissue. Donor subject (5 ft 2 in) weighed 128 lbs (58.2 kgs) at the time of the procedure.This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 59% of the cells observed at passage 5 showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN.

PDL at Senescence

PDL at Freeze

4.85

Passage Frozen

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin confirmed by LINE assay

Remark

No data is available

PDL at Senescence

Cumulative PDL at Freeze

8.31

Passage Frozen

Split Ratio

1:20

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

Adipose Stromal Cell Medium

Serum

0% fetal bovine serum Not inactivated

Substrate

Human Fibronectin

Pricing

Commercial:

$257.00USD

Academic &
Non-profit:

$103.00USD

NIA Grantees:

$47.00USD