Repository NIA Aging Cell Culture Repository
Subcollection Animals
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Family Member 1
Family History N
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 48,XX,i(5q)[2]/48,XX[48]
Species Peromyscus peromyscus polionotus
Common Name oldfield mouse
Remarks Peromyscus peromyscus polionotus (oldfield mouse); lung fibroblast culture established on 4/23/2008 from explants of minced lung tissue
PDL at Senescence 32
PDL at Freeze 3.95
Passage Frozen 4
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark Peromyscus peromyscus polionotus (oldfield mouse); lung fibroblast culture established on 4/23/2008 from explants of minced lung tissue
No data is available
No data is available
PDL at Senescence 32
Cumulative PDL at Freeze 3.95
Passage Frozen 4
Split Ratio 1:2
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM
Serum 15% fetal bovine serum Not inactivated
Substrate None specified