Repository NIA Aging Cell Culture Repository
Subcollection Animals
Biopsy Source Unspecified
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Family History N
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 49,XY,+3[16]/48,XY[34]
Species Peromyscus peromyscus californicus
Common Name California mouse
Remarks Peromyscus peromyscus californicus (California mouse); fibroblast culture established on 8/7/2008 from explants of minced skin tissue
PDL at Freeze 4.91
Passage Frozen 3
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark Peromyscus peromyscus californicus (California mouse); fibroblast culture established on 8/7/2008 from explants of minced skin tissue
No data is available
No data is available
Cumulative PDL at Freeze 4.91
Passage Frozen 3
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 3%
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate Commercially-treated plastic