GM00500
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIGMS Human Genetic Cell Repository
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Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Unspecified
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
Race
|
White
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis and Case history
|
ISCN
|
46,XY,9qh+
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
Passage Frozen |
11 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Remarks |
Skin biopsy; 46,XY,9qh+; 6% of cells show random chromosome loss |
Cencelli G, Pacini L, De Luca A, Messia I, Gentile A, Kang Y, Nobile V, Tabolacci E, Jin P, Farace MG, Bagni C, Age-Dependent Dysregulation of APP in Neuronal and Skin Cells from Fragile X Individuals Cells12: 2022 |
PubMed ID: 36899894 |
|
Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
PubMed ID: 30567591 |
|
Jones Iv AR, Coleman EL, Husni NR, Deeney JT, Raval F, Steenkamp D, Dooms H, Nikolajczyk BS, Corkey BE, Type 1 diabetes alters lipid handling and metabolism in human fibroblasts and peripheral blood mononuclear cells PloS one12:e0188474 2017 |
PubMed ID: 29206239 |
|
Russanova VR, Hirai TH, Howard BH, Semirandom sampling to detect differentiation-related and age-related epigenome remodeling The journals of gerontology Series A, Biological sciences and medical sciences59:1221-33 2005 |
PubMed ID: 15699521 |
|
Sanford KK, Parshad R, Price FM, Tarone RE, Benedict WF, Cytogenetic responses to G2 phase x-irradiation of cells from retinoblastoma patients. Cancer Genet Cytogenet88:43-8 1996 |
PubMed ID: 8630978 |
|
Coppola G, Yan Y, Hantzopoulos P, Segura E, Stroh JG, Calhoun DH, Characterization of glycosylated and catalytically active recombinant human alpha-galactosidase A using a baculovirus vector. Gene144:197-203 1994 |
PubMed ID: 8039705 |
|
Wilson R, Tocher DR, Sargent JR, Effects of exogenous monounsaturated fatty acids on fatty acid metabolism in cultured skin fibroblasts from adrenoleukodystrophy patients. J Neurol Sci109:207-14 1992 |
PubMed ID: 1634904 |
|
Hotamisligil GS, Breakefield XO, Human monoamine oxidase A gene determines levels of enzyme activity. Am J Hum Genet49:383-92 1991 |
PubMed ID: 1678250 |
|
Sims KB, Ozelius L, Corey T, Rinehart WB, Liberfarb R, Haines J, Chen WJ, Norio R, Sankila E, de la Chapelle A, et al, Norrie disease gene is distinct from the monoamine oxidase genes. Am J Hum Genet45:424-34 1989 |
PubMed ID: 2773935 |
|
Nagasawa H, Burke MJ, Little FF, McCone EF, Chan GL, Little JB, Multiple abnormalities in the ultraviolet light response of cultured fibroblasts derived from patients with the basal cell nevus syndrome. Teratog Carcinog Mutagen8:25-33 1988 |
PubMed ID: 2897722 |
|
Nagasawa H, Little JB, Radiosensitivities of ten apparently normal human diploid fibroblast strains to cell killing, G2-phase chromosomal aberrations, and cell cycle delay. Cancer Res48:4535-8 1988 |
PubMed ID: 3396006 |
|
Harper GS, Hascall VC, Yanagishita M, Gahl WA, Proteoglycan synthesis in normal and Lowe syndrome fibroblasts. J Biol Chem262:5637-43 1987 |
PubMed ID: 3571227 |
|
Nove J, Little JB, Mayer PJ, Troilo P, Nichols WW, Hypersensitivity of cells from a new chromosomal-breakage syndrome to DNA-damaging agents. Mutat Res163:255-62 1986 |
PubMed ID: 3785261 |
|
Parshad R, Sanford KK, Jones GM, Chromosomal radiosensitivity during the G2 cell-cycle period of skin fibroblasts from individuals with familial cancer. Proc Natl Acad Sci U S A82:5400-3 1985 |
PubMed ID: 3860870 |
|
Vitkauskas G, Canellakis ES, Quantitation of metabolic cooperation by measurement of HGPRTase activity. Exp Cell Res156:537-43 1985 |
PubMed ID: 2578405 |
|
Parshad R, Gantt R, Sanford KK, Jones GM, Chromosomal radiosensitivity of human tumor cells during the G2 cell cycle period. Cancer Res44:5577-82 1984 |
PubMed ID: 6498819 |
|
Vitkauskas G, Canellakis ES, The regulation of hypoxanthine guanine phosphoribosyl transferase activity through transfer of PRPP by metabolic cooperation. Exp Cell Res152:541-51 1984 |
PubMed ID: 6202536 |
|
Parshad R, Sanford KK, Jones GM, Chromatid damage after G2 phase x-irradiation of cells from cancer-prone individuals implicates deficiency in DNA repair. Proc Natl Acad Sci U S A80:5612-6 1983 |
PubMed ID: 6577447 |
|
Thielmann HW, Fischer E, Dzarlieva RT, Komitowski D, Popanda O, Edler L, Spontaneous in vitro malignant transformation in a xeroderma pigmentosum fibroblast line. Int J Cancer31:687-700 1983 |
PubMed ID: 6862680 |
|
Davis MH, Pato CN, Grinvalsky H, Gruenstein E, Identification of a biochemical difference between male and female human fibroblasts: implications for the expression of Duchenne muscular dystrophy. Neurology32:951-7 1982 |
PubMed ID: 7202167 |
|
Kurz JB, Perkins JP, Cystic fibrosis fibroblasts respond normally to isoproterenol. Pediatr Res15:1328-33 1981 |
PubMed ID: 6170925 |
|
Kurz JB, Perkins JP, Buchwald M, Killing of fibroblasts by dexamethasone or dibutyryl adenosine 3',5'- monophosphate is not a valid test for cystic fibrosis. Science206:1317-9 1979 |
PubMed ID: 229552 |
Passage Frozen |
11 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
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