Repository NIGMS Human Genetic Cell Repository
Subcollection Heritable Diseases
Class Other Disorders of Known Biochemistry
Quantity 50 µg
Quantitation Method Please see our FAQ
Cell Type Fibroblast
Transformant Untransformed
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN arr(1-22)x2,(XY)x1
Species Homo sapiens
Common Name Human
Remarks Clinically affected
Passage Frozen 6
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis
Remark Clinically affected
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013
PubMed ID: 23665875
Ling JQ, Hou A, Hoffman AR, Long-range DNA interactions are specifically altered by locked nucleic acid-targeting of a CTCF binding site Biochimica et biophysica acta1809:24-33 2010
PubMed ID: 21111075
Hjelle B, Liu E, Bishop JM, Oncogene v-src transforms and establishes embryonic rodent fibroblasts but not diploid human fibroblasts. Proc Natl Acad Sci U S A85:4355-9 1988
PubMed ID: 3132710
Schwenn MR, Weichselbaum RR, Little JB, Investigation of the cytotoxic effects of DNA damaging agents on neurofibromatosis cells. Mutat Res142:55-8 1985
PubMed ID: 3919286
Paterson, Gamma-ray hypersensitivity and faulty DNA repair in cultured cells from humans exhibiting familial cancer proneness. Proc 6th Int Cong Rad Res, Tokyo(1979) p 484:55-8 1979
PubMed ID: 3919286
No data is available
Passage Frozen 6
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA