Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN 46,XX
Species Homo sapiens
Common Name Human
Remarks 46,XX; formerly GM00237; skin biopsy (left forearm); 14% of cells show random chromosome loss/gain and 2% show random chromosomal aberrations
Passage Frozen 9
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis
 
Remark 46,XX; formerly GM00237; skin biopsy (left forearm); 14% of cells show random chromosome loss/gain and 2% show random chromosomal aberrations
Miller JD, Ganat YM, Kishinevsky S, Bowman RL, Liu B, Tu EY, Mandal PK, Vera E, Shim JW, Kriks S, Taldone T, Fusaki N, Tomishima MJ, Krainc D, Milner TA, Rossi DJ, Studer L., Human iPSC-Based Modeling of Late-Onset Disease via Progerin-Induced Aging. Cell Stem Cell.13(6):691-705 2013
PubMed ID: 24315443
 
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PubMed ID: 19800404
 
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PubMed ID: 12538859
 
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PubMed ID: 12958183
 
Laderman KA, Penny JR, Mazzucchelli F, Bresolin N, Scarlato G, Attardi G, Aging-dependent functional alterations of mitochondrial DNA (mtDNA) from human fibroblasts transferred into mtDNA-less cells. J Biol Chem271(27):15891-7 1996
PubMed ID: 8663253
 
Nette EG, Sit HL, King DW, Reactivation of DNA synthesis in aging diploid human skin fibroblasts by fusion with mouse L karyoplasts cytoplasts and whole L cells. Mech Ageing Dev18:75-87 1982
PubMed ID: 7062789
 
Hall JD, Almy RE, Scherer KL, DNA repair in cultured human fibroblasts does not decline with donor age. Exp Cell Res139:351-9 1982
PubMed ID: 6282605
 
Slayback JR, Cheung LW, Geyer RP, Comparative effects of human platelet growth factor on the growth and morphology of human fetal and adult diploid fibroblasts. Exp Cell Res110:462-6 1977
PubMed ID: 590364
No data is available
Passage Frozen 9
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA