Repository NIGMS Human Genetic Cell Repository
Subcollection CEPH
Repository Linkage Families
PIGI Consented Sample
Quantity 0.050mg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Caucasian
Family Member 10
Relation to Proband paternal grandfather
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Paternal Grandfather
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME PCR analysis of DNA from this cell culture gave a positive result with a primer for Yq11, DYS227.
Remark Paternal Grandfather
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PubMed ID: 16826523
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PubMed ID: 15747258
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PubMed ID: 15185041
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PubMed ID: 11951176
Bowman ED, Bromeke B, Lensing W, Shields PG, Apolipoprotein E allelic frequency in elderly smokers. Am J Med Genet76(1):32-6 1998
PubMed ID: 9508061
Haaf T, Willard HF, Organization, polymorphism, and molecular cytogenetics of chromosome- specific alpha-satellite DNA from the centromere of chromosome 2. Genomics13:122-8 1992
PubMed ID: 1577477
Nicholls RD, Knoll JH, Glatt K, Hersh JH, Brewster TD, Graham JM Jr, Wurster-Hill D, Wharton R, Latt SA, Restriction fragment length polymorphisms within proximal 15q and their use in molecular cytogenetics and the Prader-Willi syndrome. Am J Med Genet33:66-77 1989
PubMed ID: 2568752
Simon M, Phillips M, Green H, Stroh H, Glatt K, Burns G, Latt SA, Absence of a single repeat from the coding region of the human involucrin gene leading to RFLP. Am J Hum Genet45:910-6 1989
PubMed ID: 2574003
Board PG, Chapple R, Coggan M, Haplotypes of the coagulation factor XIII A subunit locus in normal and deficient subjects. Am J Hum Genet42:712-7 1988
PubMed ID: 2895980
Buroker NE, Magenis RE, Weliky K, Bruns G, Litt M, Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum Genet72:86-94 1986
PubMed ID: 3002956
Bufton L, Bruns GA, Magenis RE, Tomar D, Shaw D, Brook D, Litt M, Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. Am J Hum Genet38:447-60 1986
PubMed ID: 3010711
No data is available
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium