GM10792
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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12
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster hybrid retaining human chromosomes #12 (100% of cells) & #19 (92% of cells)
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xq28, DXS15, and Xp22.32 and Yp11.3, MIC2. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 8p22p12, NFL. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 9q34, D9S7. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 12p13.2, PRB1. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q12.3, D18S6. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 19q13.3, LHB. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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Remarks |
Line 5HL2-B; produced by fusing normal human lymphocytes & thioguanine-resistant & HPRT-deficient CHO line UV5; selected on the basis of resistance to UV irradiation; retains human chromosomes #12 (100% of cells) & #19 (92% of cells) |
Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
PubMed ID: 9479491 |
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Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
PubMed ID: 7601467 |
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MacCollin M, Peterfreund R, MacDonald M, Fink JS, Gusella J, Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22. Genomics20:332-3 1994 |
PubMed ID: 8020991 |
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Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
PubMed ID: 8275700 |
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Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
PubMed ID: 8402913 |
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Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
PubMed ID: 8478002 |
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Thompson LH, Bachinski LL, Stallings RL, Dolf G, Weber CA, Westerveld A, Siciliano MJ, Complementation of repair gene mutations on the hemizygous chromosome 9 in CHO: a third repair gene on human chromosome 19. Genomics5:670-9 1989 |
PubMed ID: 2591959 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
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