GM11686
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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2
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/mouse hybrid retaining human chromosome #2 in 100% of cells
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xp22.32 and Yp11.3, MIC2, and Xq28, DXS15. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 2p25, D2S1. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10q24qter, PLAU. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. |
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Remarks |
Line HA(2)A; produced by the transfer of neo-marked human chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium containing G418; retains human chromosome #2 in 100% of cells |
Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
PubMed ID: 16537608 |
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Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997 |
PubMed ID: 9126495 |
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Longo N, Langley SD, Griffin LD, Elsas LJ, Two mutations in the insulin receptor gene of a patient with leprechaunism: application to prenatal diagnosis. J Clin Endocrinol Metab80(5):1496-501 1995 |
PubMed ID: 1438292 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
8% |
Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Geneticin 500ug/ml |
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