GM13260
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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20
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human chromosome #20
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 20p12, D20S6. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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Remarks |
Line A9+20; produced by the transfer of neo-marked human, fetal, female, lung fibroblast chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium with G418; retains human chromo #20 in 69% of cells |
Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
PubMed ID: 16537608 |
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Tiranti V, Hoertnagel K, Carrozzo R, Galimberti C, Munaro M, Granatiero M, Zelante L, Gasparini P, Marzella R, Rocchi M, Bayona-Bafaluy MP, Enriquez JA, Uziel G, Bertini E, Dionisi-Vici C, Franco B, Meitinger T, Zeviani M, Mutations of SURF-1 in leigh disease associated with cytochrome c oxidase deficiency [In Process Citation] Am J Hum Genet63:1609-21 1998 |
PubMed ID: 9837813 |
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Whitney M, Thayer M, Reifsteck C, Olson S, Smith L, Jakobs PM, Leach R, Naylor S, Joenje H, Grompe M, Microcell mediated chromosome transfer maps the Fanconi anaemia group D gene to chromosome 3p. Nat Genet11:341-3 1995 |
PubMed ID: 7581463 |
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Ning Y, Lovell M, Cooley LD, Pereira-Smith OM, "PCR karyotype" of monochromosomal somatic cell hybrids. Genomics16:758-60 1993 |
PubMed ID: 8325652 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Geneticin 250ug/ml |
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