GM14972
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retaining human der(16)t(4;16)(q31;p13.3) (92%) and #2, 14, 17, 20, and 21
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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Remarks |
Line CY14; produced by the fusion of human cells with karyotype t(4;16) (q31.1;p13.3) with A9 mouse cells; grown in medium with azaserine & adenine; retains a human der(16)t(4;16)(q31;p13.3) in 92% of cells & other human chromos |
Bailey JA, Yavor AM, Viggiano L, Misceo D, Horvath JE, Archidiacono N, Schwartz S, Rocchi M, Eichler EE, Human-specific duplication and mosaic transcripts: the recent paralogous structure of chromosome 22. Am J Hum Genet70(1):83-100 2002 |
PubMed ID: 11731936 |
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Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990 |
PubMed ID: 2095701 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Adenine 5 x 10-2mM |
Supplement |
Azaserine 5 x 10-2mM |
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