GM15993
LCL from B-Lymphocyte
Description:
TRANSLOCATED CHROMOSOME
BREAST CANCER
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Class |
Disorders of Uncertain Biochemical Etiology |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Family Member
|
2
|
Relation to Proband
|
maternal aunt
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XX,t(11;12)(11pter>11q24::12q22>12qter;12pter>12q22::11q24>11qter)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Cytogenetics |
Chromosome 11: TRANSLOCATION Breakpoint 11q24 t(11;12)11q24 |
|
Chromosome 12: TRANSLOCATION Breakpoint 12q22 t(11;12)12q22 |
Remarks |
Breast cancer diagnosed at age 42; niece also diagnosed with breast cancer at age 42 (GM15232) |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|