GM19625
LCL from B-Lymphocyte
Description:
INTERNATIONAL HAPMAP PROJECT - AFRICAN ANCESTRY IN SOUTHWEST USA
INTERNATIONAL HAPMAP PROJECT - PLATE OF AFRICAN ANCESTRY IN SOUTHWEST USA
1000 GENOMES PROJECT - PANEL OF 62 AFRICAN ANCESTRY IN SOUTHWEST USA
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Repository
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NHGRI Sample Repository for Human Genetic Research
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| Subcollection |
Human Variation |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Ethnicity
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AFRICAN ANCESTRY
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Country of Origin
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USA
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Family Member
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1
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Relation to Proband
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child
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remark |
At least three out of four grandparents identified themselves as African-American |
| Shen P, Wang W, Krishnakumar S, Palm C, Chi AK, Enns GM, Davis RW, Speed TP, Mindrinos MN, Scharfe C, High-quality DNA sequence capture of 524 disease candidate genes Proceedings of the National Academy of Sciences of the United States of America108:6549-54 2011 |
| PubMed ID: 21467225 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
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