GM19625
LCL from B-Lymphocyte
Description:
INTERNATIONAL HAPMAP PROJECT - AFRICAN ANCESTRY IN SOUTHWEST USA
INTERNATIONAL HAPMAP PROJECT - PLATE OF AFRICAN ANCESTRY IN SOUTHWEST USA
1000 GENOMES PROJECT - PANEL OF 62 AFRICAN ANCESTRY IN SOUTHWEST USA
Repository
|
NHGRI Sample Repository for Human Genetic Research
|
Subcollection |
Human Variation |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Ethnicity
|
AFRICAN ANCESTRY
|
Country of Origin
|
USA
|
Family Member
|
1
|
Relation to Proband
|
child
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remark |
At least three out of four grandparents identified themselves as African-American |
Shen P, Wang W, Krishnakumar S, Palm C, Chi AK, Enns GM, Davis RW, Speed TP, Mindrinos MN, Scharfe C, High-quality DNA sequence capture of 524 disease candidate genes Proceedings of the National Academy of Sciences of the United States of America108:6549-54 2011 |
PubMed ID: 21467225 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
|
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