GM21143
LCL from B-Lymphocyte
Description:
INTERNATIONAL HAPMAP PROJECT - GUJARATI INDIANS IN HOUSTON, TEXAS, USA
INTERNATIONAL HAPMAP PROJECT - PANEL OF 90 GUJARATI INDIANS IN HOUSTON, TEXAS, USA
1000 GENOMES PROJECT - PANEL OF 109 GUJARATI
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Repository
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NHGRI Sample Repository for Human Genetic Research
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| Subcollection |
Human Variation |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Ethnicity
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GUJARATI INDIAN
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Country of Origin
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USA
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Relation to Proband
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proband
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
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| Remarks |
At least three out of four grandparents identified themselves as Gujarati; sample collected from an individual in the Houston, Texas metropolitan area |
| Holt CL, Stephens KM, Walichiewicz P, Fleming KD, Forouzmand E, Wu SF, Human Mitochondrial Control Region and mtGenome: Design and Forensic Validation of NGS Multiplexes, Sequencing and Analytical Software Genes12: 2020 |
| PubMed ID: 33921728 |
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| McCabe MJ, Gauthier MA, Chan CL, Thompson TJ, De Sousa SMC, Puttick C, Grady JP, Gayevskiy V, Tao J, Ying K, Cipponi A, Deng N, Swarbrick A, Thomas ML, Lord RV, Johns AL, Kohonen-Corish M, O'Toole SA, Clark J, Mueller SA, Gupta R, McCormack AI, Dinger ME, Cowley MJ, kConFab MJ, Development and validation of a targeted gene sequencing panel for application to disparate cancers Scientific reports9:17052 2019 |
| PubMed ID: 31745186 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
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