Description:
HTT-Q23-PUC, MIX, 1-3144, HUMAN
HUNTINGTON DISEASE; HD
|
Repository
|
HD Community Biorepository
|
| Quantity |
20 µg |
| Quantitation Method |
Please see our FAQ |
| Certain Third Party Licensing Requirements |
Blue Heron pBase |
|
Alias
|
FLmixQ23
|
|
Sample Source
|
CHDI Foundation, Inc.
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| Remarks |
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n |
| Construct Name |
FLmixQ23 |
| Insert Length |
9454 |
| Vector |
pHTTbase |
| Concentration |
75 ng/ml |
| Sequence |
1 CTTTCACTGA CTGACTGACT GGAAGACACA CTCATGCGTT TAAACACCAC
CATGGCGACC CTGGAAAAGC TGATGAAGGC CTTCGAGTCC CTCAAGTCCT
101 TCCAACAGCA GCAACAGCAA CAACAGCAGC AACAGCAACA ACAGCAGCAA
CAGCAACAAC AGCAGCAACA GCCGCCACCG CCGCCGCCGC CGCCGCCGCC
2
01 TCCTCAGCTT CCTCAGCCGC CGCCGCAGGC ACAGCCGCTG CTGCCTCAGC C
GCAGCCGCC CCCGCCGCCG CCCCCGCCGC CACCCGGCCC GGCTGTGGCT
30
1 GAGGAGCCGC TGCACCGACC AAAGAAAGAA CTTTCAGCTA CCAAGAAAGA CC
GTGTGAAT CATTGTCTGA CAATATGTGA AAACATAGTG GCACAGTCTG
401
TCAGAAATTC TCCAGAATTT CAGAAACTTC TGGGCATCGC TATGGAACTT TTT
CTGCTGT GCAGTGATGA CGCAGAGTCA GATGTCAGGA TGGTGGCTGA
501
CGAATGCCTC AACAAAGTTA TCAAAGCTTT GATGGATTCT AATCTTCCAA GGTT
ACAGCT CGAGCTCTAT AAGGAAATTA AAAAGAATGG TGCCCCTCGG
601 A
GTTTGCGTG CTGCCCTGTG GAGGTTTGCT GAGCTGGCTC ACCTGGTTCG GCCTC
AGAAA TGCAGGCCTT ACCTGGTGAA CCTTCTGCCG TGCCTGACTC
701 GA
ACAAGCAA GAGACCCGAA GAATCAGTCC AGGAGACCTT GGCTGCAGCT GTTCCC
AAAA TTATGGCTTC TTTTGGCAAT TTTGCAAATG ACAATGAAAT
801 TAA
GGTTTTG TTAAAGGCCT TCATAGCGAA CCTGAAGTCA AGCTCCCCCA CCATTCG
GCG GACAGCGGCT GGATCAGCAG TGAGCATCTG CCAGCACTCA
901 AGAA
GGACAC AATATTTCTA TAGTTGGCTA CTAAATGTGC TCTTAGGCTT ACTCGTTC
CT GTCGAGGATG AACACTCCAC TCTGCTGATT CTTGGCGTGC
1001 TGCTC
ACCCT GAGGTATTTG GTGCCCTTGC TGCAGCAGCA GGTCAAGGAC ACAAGCCTG
A AAGGCAGCTT CGGAGTGACA AGGAAAGAAA TGGAAGTCTC
1101 TCCTTC
TGCA GAGCAGCTTG TCCAGGTTTA TGAACTGACG TTACATCATA CACAGCACCA
AGACCACAAT GTTGTGACCG GAGCCCTGGA GCTGTTGCAG
1201 CAGCTCT
TCA GAACGCCTCC ACCCGAGCTT CTGCAAACCC TGACCGCAGT CGGGGGCATT
GGGCAGCTCA CCGCTGCTAA GGAGGAGTCT GGTGGCCGAA
1301 GCCGTAGT
GG GAGTATTGTG GAACTTATAG CTGGAGGGGG TTCCTCATGC AGCCCTGTCC T
TTCAAGAAA ACAAAAAGGC AAAGTGCTCT TAGGAGAAGA
1401 AGAAGCCTT
G GAGGATGACT CTGAATCGAG ATCGGATGTC AGCAGCTCTG CCTTAACAGC CT
CAGTGAAG GATGAGATCA GTGGAGAGCT GGCTGCTTCT
1501 TCAGGGGTTT
CCACTCCAGG GTCAGCAGGT CATGACATCA TCACAGAACA GCCACGGTCA CAG
CACACAC TGCAGGCGGA CTCAGTGGAT CTGGCCAGCT
1601 GTGACTTGAC
AAGCTCTGCC ACTGATGGGG ATGAGGAGGA TATCTTGAGC CACAGCTCCA GCCA
GGTCAG CGCCGTCCCA TCTGACCCTG CCATGGACCT
1701 GAATGATGGG A
CCCAGGCCT CGTCGCCCAT CAGCGACAGC TCCCAGACCA CCACCGAAGG GCCTG
ATTCA GCTGTTACCC CTTCAGACAG TTCTGAAATT
1801 GTGTTAGACG GT
ACCGACAA CCAGTATTTG GGCCTGCAGA TTGGACAGCC CCAGGATGAA GATGAG
GAAG CCACAGGTAT TCTTCCTGAT GAAGCCTCGG
1901 AGGCCTTCAG GAA
CTCTTCC ATGGCCCTTC AACAGGCACA TTTATTGAAA AACATGAGTC ACTGCAG
GCA GCCTTCTGAC AGCAGTGTTG ATAAATTTGT
2001 GTTGAGAGAT GAAG
CTACTG AACCGGGTGA TCAAGAAAAC AAGCCTTGCC GCATCAAAGG TGACATTG
GA CAGTCCACTG ATGATGACTC TGCACCTCTT
2101 GTCCATTGTG TCCGC
CTTTT ATCTGCTTCG TTTTTGCTAA CAGGGGGAAA AAATGTGCTG GTTCCGGAC
A GGGATGTGAG GGTCAGCGTG AAGGCCCTGG
2201 CCCTCAGCTG TGTGGG
AGCA GCTGTGGCCC TCCACCCGGA ATCTTTCTTC AGCAAACTCT ATAAAGTTCC
TCTTGACACC ACGGAATACC CTGAGGAACA
2301 GTATGTCTCA GACATCT
TGA ACTACATCGA TCATGGAGAC CCACAGGTTC GAGGAGCCAC TGCCATTCTC
TGTGGGACCC TCATCTGCTC CATCCTCAGC
2401 AGGTCCCGCT TCCACGTG
GG AGATTGGATG GGCACCATTA GAACCCTCAC AGGAAATACA TTTTCTTTGG C
GGATTGCAT TCCTTTGCTG CGGAAAACAC
2501 TGAAGGATGA GTCTTCTGT
T ACTTGCAAGT TAGCTTGTAC AGCTGTGAGG AACTGTGTCA TGAGTCTCTG CA
GCAGCAGC TACAGTGAGT TAGGACTGCA
2601 GCTGATCATC GATGTGCTGA
CTCTGAGGAA CAGTTCCTAT TGGCTGGTGA GGACAGAGCT TCTGGAAACC CTT
GCAGAGA TTGACTTCAG GCTGGTGAGC
2701 TTTTTGGAGG CAAAAGCAGA
AAACTTACAC AGAGGGGCTC ATCATTATAC AGGGCTTTTA AAACTGCAAG AACG
AGTGCT CAATAATGTT GTCATCCATT
2801 TGCTTGGAGA TGAAGACCCC A
GGGTGCGAC ATGTTGCCGC AGCATCACTA ATTAGGCTTG TCCCAAAGCT GTTTT
ATAAA TGTGACCAAG GACAAGCTGA
2901 TCCAGTAGTG GCCGTGGCAA GA
GATCAAAG CAGTGTTTAC CTGAAACTTC TCATGCATGA AACGCAGCCT CCATCT
CATT TCTCCGTCAG CACAATAACC
3001 AGAATATATA GAGGCTATAA CCT
ACTACCA AGCATAACAG ACGTCACTAT GGAAAATAAC CTTTCAAGAG TTATTGC
AGC AGTTTCTCAT GAACTAATCA
3101 CATCAACCAC CAGAGCACTC ACAT
TTGGAT GCTGTGAAGC TTTGTGTCTT CTTTCCACTG CCTTCCCAGT TTGCATTT
GG AGTTTAGGTT GGCACTGTGG
3201 AGTGCCTCCA CTGAGTGCCT CAGAT
GAGTC TAGGAAGAGC TGTACCGTTG GGATGGCCAC AATGATTCTG ACCCTGCTC
T CGTCAGCTTG GTTCCCATTG
3301 GATCTCTCAG CCCATCAAGA TGCTTT
GATT TTGGCCGGAA ACTTGCTTGC AGCCAGTGCT CCCAAATCTC TGAGAAGTTC
ATGGGCCTCT GAAGAAGAAG
3401 CCAACCCAGC AGCCACCAAG CAAGAGG
AGG TCTGGCCAGC CCTGGGGGAC CGGGCCCTGG TGCCCATGGT GGAGCAGCTC
TTCTCTCACC TGCTGAAGGT
3501 GATTAACATT TGTGCCCACG TCCTGGAT
GA CGTGGCTCCT GGACCCGCAA TAAAGGCAGC CTTGCCTTCT CTAACAAACC C
CCCTTCTCT AAGTCCCATC
3601 CGACGAAAGG GGAAGGAGAA AGAACCAGG
A GAACAAGCAT CTGTACCGTT GAGTCCCAAG AAAGGCAGTG AGGCCAGTGC AG
CTTCTAGA CAATCTGATA
3701 CCTCAGGTCC TGTTACAACA AGTAAATCCT
CATCACTGGG GAGTTTCTAT CATCTTCCTT CATACCTCAA ACTGCATGAT GTC
CTGAAAG CTACACACGC
3801 TAACTACAAG GTCACGCTGG ATCTTCAGAA
CAGCACGGAA AAGTTTGGAG GGTTTCTCCG CTCAGCCTTG GATGTTCTTT CTCA
GATACT AGAGCTGGCC
3901 ACACTGCAGG ACATTGGGAA GTGTGTTGAA G
AGATCCTAG GATACCTGAA ATCCTGCTTT AGTCGAGAAC CAATGATGGC AACTG
TTTGT GTTCAACAAT
4001 TGTTGAAGAC TCTCTTTGGC ACAAACTTGG CC
TCCCAGTT TGATGGCTTA TCTTCCAACC CCAGCAAGTC ACAAGGCCGA GCACAG
CGCC TTGGCTCCTC
4101 CAGTGTGAGG CCAGGCTTGT ACCACTACTG CTT
CATGGCC CCGTACACCC ACTTCACCCA GGCCCTCGCT GACGCCAGCC TGAGGAA
CAT GGTGCAGGCG
4201 GAGCAGGAGA ACGACACCTC GGGATGGTTT GATG
TCCTCC AGAAAGTGTC TACCCAGTTG AAGACAAACC TCACGAGTGT CACAAAGA
AC CGTGCAGATA
4301 AGAATGCTAT TCATAATCAC ATTCGTTTGT TTGAA
CCTCT TGTTATAAAA GCTTTAAAAC AGTACACGAC TACAACATGT GTGCAGTTA
C AGAAGCAGGT
4401 TTTAGATTTG CTGGCGCAGC TGGTTCAGTT ACGGGT
TAAT TACTGTCTTC TGGATTCAGA TCAGGTGTTT ATTGGCTTTG TATTGAAACA
GTTTGAATAC
4501 ATTGAAGTGG GCCAGTTCAG GGAATCAGAG GCAATCA
TTC CAAACATCTT TTTCTTCTTG GTATTACTAT CTTATGAACG CTATCATTCA
AAACAGATCA
4601 TTGGAATTCC TAAAATCATT CAGCTCTGTG ATGGCATC
AT GGCCAGTGGA AGGAAGGCTG TGACACATGC CATACCGGCT CTGCAGCCCA T
AGTCCACGA
4701 CCTCTTTGTA TTAAGAGGAA CAAATAAAGC TGATGCAGG
A AAAGAGCTTG AAACCCAAAA AGAGGTGGTG GTGTCAATGT TACTGAGACT CA
TCCAGTAC
4801 CATCAGGTGT TGGAGATGTT CATTCTTGTC CTGCAGCAGT
GCCACAAGGA GAATGAAGAC AAGTGGAAGC GACTGTCTCG ACAGATAGCT GAC
ATCATCC
4901 TCCCAATGTT AGCCAAACAG CAGATGCACA TTGACTCTCA
TGAAGCCCTT GGAGTGTTAA ATACATTATT TGAGATTTTG GCCCCTTCCT CCCT
CCGTCC
5001 GGTAGACATG CTTTTACGGA GTATGTTCGT CACTCCAAAC A
CAATGGCGT CCGTGAGCAC TGTTCAACTG TGGATATCGG GAATTCTGGC CATTT
TGAGG
5101 GTTCTGATTT CCCAGTCAAC TGAAGATATT GTTCTTTCTC GT
ATTCAGGA GCTCTCCTTC TCTCCGTATT TAATCTCCTG TACAGTAATT AATAGG
TTAA
5201 GAGATGGGGA CAGTACTTCA ACGCTAGAAG AACACAGTGA AGG
GAAACAA ATAAAGAATT TGCCAGAAGA AACATTTTCA AGGTTTCTAT TACAACT
GGT
5301 TGGTATTCTT TTAGAAGACA TTGTTACAAA ACAGCTGAAG GTGG
AAATGA GTGAGCAGCA ACATACTTTC TATTGCCAGG AACTAGGCAC ACTGCTAA
TG
5401 TGTCTGATCC ACATCTTCAA GTCTGGAATG TTCCGGAGAA TCACA
GCAGC TGCCACTAGG CTGTTCCGCA GTGATGGCTG TGGCGGCAGT TTCTACACC
C
5501 TGGACAGCTT GAACTTGCGG GCTCGTTCCA TGATCACCAC CCACCC
GGCC CTGGTGCTGC TCTGGTGTCA GATACTGCTG CTTGTCAACC ACACCGACTA
5601 CCGCTGGTGG GCAGAAGTGC AGCAGACCCC GAAAAGACAC AGTCTGT
CCA GCACAAAGTT ACTTAGTCCC CAGATGTCTG GAGAAGAGGA GGATTCTGAC
5701 TTGGCAGCCA AACTTGGAAT GTGCAATAGA GAAATAGTAC GAAGAGGG
GC TCTCATTCTC TTCTGTGATT ATGTCTGTCA GAACCTCCAT GACTCCGAGC
5801 ACTTAACGTG GCTCATTGTA AATCACATTC AAGATCTGAT CAGCCTTTC
C CACGAGCCTC CAGTACAGGA CTTCATCAGT GCCGTTCATC GGAACTCTGC
5901 TGCCAGCGGC CTGTTCATCC AGGCAATTCA GTCTCGTTGT GAAAACCTTT
CAACTCCAAC CATGCTGAAG AAAACTCTTC AGTGCTTGGA GGGGATCCAT
6
001 CTCAGCCAGT CGGGAGCTGT GCTCACGCTG TATGTGGACA GGCTTCTGTG
CACCCCTTTC CGTGTGCTGG CTCGCATGGT CGACATCCTT GCTTGTCGCC
61
01 GGGTAGAAAT GCTTCTGGCT GCAAATTTAC AGAGCAGCAT GGCCCAGTTG C
CAATGGAAG AACTCAACAG AATCCAGGAA TACCTTCAGA GCAGCGGGCT
620
1 CGCTCAGAGA CACCAAAGGC TCTATTCCCT GCTGGACAGG TTTCGTCTGT CC
ACCATGCA AGACTCACTT AGTCCCTCTC CTCCAGTCTC TTCCCACCCG
6301
CTGGACGGGG ATGGGCACGT GTCACTGGAA ACAGTGAGTC CGGACAAAGA CTG
GTACGTT CATCTTGTCA AATCCCAGTG TTGGACCAGG TCAGATTCTG
6401
CACTGCTGGA AGGTGCAGAG CTGGTGAATC GGATTCCTGC TGAAGATATG AATG
CCTTCA TGATGAACTC GGAGTTCAAC CTAAGCCTGC TAGCTCCATG
6501 C
TTAAGCCTA GGGATGAGTG AAATTTCTGG TGGCCAGAAG AGTGCCCTTT TTGAA
GCAGC CCGTGAGGTG ACTCTGGCCC GTGTGAGCGG CACCGTGCAG
6601 CA
GCTCCCTG CTGTCCATCA TGTCTTCCAG CCCGAGCTGC CTGCAGAGCC GGCGGC
CTAC TGGAGCAAGT TGAATGATCT GTTTGGGGAT GCTGCACTGT
6701 ATC
AGTCCCT GCCCACTCTG GCCCGGGCCC TGGCACAGTA CCTGGTGGTG GTCTCCA
AAC TGCCCAGTCA TTTGCACCTT CCTCCTGAGA AAGAGAAGGA
6801 CATT
GTGAAA TTCGTGGTGG CAACCCTTGA GGCCCTGTCC TGGCATTTGA TCCATGAG
CA GATCCCGCTG AGTCTGGATC TCCAGGCAGG GCTGGACTGC
6901 TGCTG
CCTGG CCCTGCAGCT GCCTGGCCTC TGGAGCGTGG TCTCCTCCAC AGAGTTTGT
G ACCCACGCCT GCTCCCTCAT CTACTGTGTG CACTTCATCC
7001 TGGAGG
CCGT TGCAGTGCAG CCTGGAGAGC AGCTTCTTAG TCCAGAAAGA AGGACAAATA
CCCCAAAAGC CATCAGCGAG GAGGAGGAGG AAGTAGATCC
7101 AAACACA
CAG AATCCTAAGT ATATCACTGC AGCCTGTGAG ATGGTGGCAG AAATGGTGGA
GTCTCTGCAG TCGGTGTTGG CCTTGGGTCA TAAAAGGAAT
7201 AGCGGCGT
GC CGGCGTTTCT CACGCCATTG CTCAGGAACA TCATCATCAG CCTGGCCCGC C
TGCCCCTTG TCAACAGCTA CACACGTGTG CCCCCACTGG
7301 TGTGGAAGC
T TGGATGGTCA CCCAAACCGG GAGGGGATTT TGGCACAGCA TTCCCTGAGA TC
CCCGTGGA GTTCCTCCAG GAAAAGGAAG TCTTTAAGGA
7401 GTTCATCTAC
CGCATCAACA CACTAGGCTG GACCAGTCGT ACTCAGTTTG AAGAAACTTG GGC
CACCCTC CTTGGTGTCC TGGTGACGCA GCCCCTCGTG
7501 ATGGAGCAGG
AGGAGAGCCC ACCAGAAGAA GACACAGAGA GGACCCAGAT CAACGTCCTG GCCG
TGCAGG CCATCACCTC ACTGGTGCTC AGTGCAATGA
7601 CTGTGCCTGT G
GCCGGCAAC CCAGCTGTAA GCTGCTTGGA GCAGCAGCCC CGGAACAAGC CTCTG
AAAGC TCTCGACACC AGGTTTGGGA GGAAGCTGAG
7701 CATTATCAGA GG
GATTGTGG AGCAAGAGAT TCAAGCAATG GTTTCAAAGA GAGAGAATAT TGCCAC
CCAT CATTTATATC AGGCATGGGA TCCTGTCCCT
7801 TCTCTGTCTC CGG
CTACTAC AGGTGCCCTC ATCAGCCACG AGAAGCTGCT GCTACAGATC AACCCCG
AGC GGGAGCTGGG GAGCATGAGC TACAAACTCG
7901 GCCAGGTGTC CATA
CACTCC GTGTGGCTGG GGAACAGCAT CACACCCCTG AGGGAGGAGG AATGGGAC
GA GGAAGAGGAG GAGGAGGCCG ACGCCCCTGC
8001 ACCTTCGTCA CCACC
CACTT CTCCAGTCAA CTCCAGGAAA CACCGGGCTG GAGTTGACAT CCACTCCTG
T TCGCAGTTTT TGCTTGAGTT GTACAGCCGC
8101 TGGATCCTGC CGTCCA
GCTC AGCCAGGAGG ACCCCGGCCA TCCTGATCAG TGAGGTGGTC AGATCCCTTC
TAGTGGTCTC AGACTTGTTC ACCGAGCGCA
8201 ACCAGTTTGA GCTGATG
TAT GTGACGCTGA CAGAACTGCG AAGGGTGCAC CCTTCAGAAG ACGAGATCCT
CGCTCAGTAC CTGGTGCCTG CCACCTGCAA
8301 GGCAGCTGCC GTCCTTGG
GA TGGACAAGGC CGTGGCGGAG CCTGTCAGCC GCCTGCTGGA GAGCACGCTC A
GGAGCAGCC ACCTGCCCAG CAGGGTTGGA
8401 GCCCTGCACG GCGTCCTCT
A TGTGCTGGAG TGCGACCTGC TGGACGACAC TGCCAAGCAG CTCATCCCGG TC
ATCAGCGA CTATCTCCTC TCCAACCTGA
8501 AAGGGATCGC CCACTGCGTG
AACATTCACA GCCAGCAGCA CGTACTGGTC ATGTGTGCCA CTGCGTTTTA CCT
CATTGAG AACTATCCTC TGGACGTAGG
8601 GCCGGAATTT TCAGCATCAA
TAATACAGAT GTGTGGGGTG ATGCTGTCTG GAAGTGAGGA GTCCACCCCC TCCA
TCATTT ACCACTGTGC CCTCAGAGGC
8701 CTGGAGCGCC TCCTGCTCTC T
GAGCAGCTC TCCCGCCTGG ATGCAGAATC GCTGGTCAAG CTGAGTGTGG ACAGA
GTGAA CGTGCACAGC CCGCACCGGG
8801 CCATGGCGGC TCTGGGCCTG AT
GCTCACCT GCATGTACAC AGGAAAGGAG AAAGTCAGTC CGGGTAGAAC TTCAGA
CCCT AATCCTGCAG CCCCCGACAG
8901 CGAGTCAGTG ATTGTTGCTA TGG
AGCGGGT ATCTGTTCTT TTTGATAGGA TCAGGAAAGG CTTTCCTTGT GAAGCCA
GAG TGGTGGCCAG GATCCTGCCC
9001 CAGTTTCTAG ACGACTTCTT CCCA
CCCCAG GACATCATGA ACAAAGTCAT CGGAGAGTTT CTGTCCAACC AGCAGCCA
TA CCCCCAGTTC ATGGCCACCG
9101 TGGTGTATAA GGTGTTTCAG ACTCT
GCACA GCACCGGGCA GTCGTCCATG GTCCGGGACT GGGTCATGCT GTCCCTCTC
C AACTTCACGC AGAGGGCCCC
9201 GGTCGCCATG GCCACGTGGA GCCTCT
CCTG CTTCTTTGTC AGCGCGTCCA CCAGCCCGTG GGTCGCGGCG ATCCTCCCAC
ATGTCATCAG CAGGATGGGC
9301 AAGCTGGAGC AGGTGGACGT GAACCTT
TTC TGCCTGGTCG CCACAGACTT CTACAGACAC CAGATAGAGG AGGAGCTCGA
CCGCAGGGCC TTCCAGTCTG
9401 TGCTTGAGGT GGTTGCAGCC CCAGGAAG
CC CATATCACCG GCTGCTGACT TGTTTACGAA ATGTCCACAA GGTCACCACC T
GCGCGGCCG CAGCTCGGCA
9501 GAGACGACTG ACTGACTGAC TGGAAAGAG
G AAGGGCTGGA AGAGGAAGGA GCTTGGCGTA ATCATGGTCA TAGCT
|
| Cloning Information |
5' Restriction Site: BsmBI, 3' Restriction Site: BsmBI |
| Remarks |
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n |
| DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n |
| Remarks |
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n |
| PDF |
Vector Map |
|
Plasmid Collection Screening and Propagation |
| Supplement |
- |
|
|