Characterization and Quality Control

Proper characterization and quality control are essential for ensuring the integrity, reliability, and safety of iPSC lines. Our comprehensive process includes:

Cell MorphologyEvaluating growth patterns of typical iPSCs
Molecular AnalysisConfirming pluripotency-associated gene expression using PCR and RNA sequencing
Growth and Viability AssessmentMonitoring cell proliferation and health
Microbial and Fungal Sterility TestingDetecting and eliminating contaminants
Mycoplasma TestingPreventing contamination by mycoplasma species
Human Pathogen TestingScreening for pathogens to ensure safety
Identity TestingMicrosatellite analysis for genetic match verification
Cytogenetic G-Banded KaryotypeEnsuring chromosomal stability
Microarray GenotypingConfirming genetic authenticity and stability
Confirmation of Integration-Free ReprogrammingVerifying no genomic integration
Stem Cell Surface Marker AssessmentUsing immunocytochemistry, flow cytometry, and alkaline phosphatase staining
In Vitro Embryoid Body (EB) AssayConfirming pluripotency through 3D aggregate testing
Pluripotency and Tri-Lineage Differentiation PotentialUsing the Applied Biosystems TaqMan hPSC Scorecard Panel to evaluate differentiation into ectoderm, mesoderm, and endoderm germ layers

Coriell’s commitment to meticulous quality control ensures that every iPSC line meets the highest standards of reliability and reproducibility.


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