Efficiently expanding and maintaining induced pluripotent stem cells (iPSCs) in culture is a critical step in harnessing their full potential. iPSC expansion refers to increasing the number of iPSCs in culture while preserving their pluripotency—the ability to differentiate into any cell type in the human body. This process is essential for applications such as drug screening, disease modeling, and regenerative medicine.
Successful expansion enables researchers to generate sufficient quantities of patient-specific cells for meaningful research. Key applications include:
| Disease Modeling | iPSCs derived from patients with genetic disorders, such as Alzheimer’s or cardiac diseases, can be expanded and differentiated into relevant cell types (e.g., neurons or cardiomyocytes). These models allow researchers to study disease progression in vitro and explore therapeutic interventions. |
| Drug Development | Expanded iPSCs facilitate high-throughput screening of drug candidates, accelerating the discovery of new treatments. Without reliable expansion methods, scaling up these applications would be impractical. |
The Coriell Institute leads the field in iPSC expansion by leveraging cutting-edge techniques and advanced culture systems to overcome these challenges. Our expertise ensures the production of high-quality iPSC lines that are reproducible, genetically stable, and scalable for a variety of applications. Our capabilities include:
| Feeder-Free Culture Systems | Optimized systems eliminate variability and improve consistency in iPSC cultures. |
| Defined Media and Matrices | Serum-free media and defined matrices enhance scalability and reproducibility. |
| Bioreactor-Based Expansion | State-of-the-art bioreactor systems provide a controlled environment for large-scale iPSC production, minimizing contamination risks and ensuring genetic stability. |
| Pluripotency Maintenance | Rigorous protocols ensure that iPSCs retain their pluripotency and differentiation potential, even during long-term culture. |
| Comprehensive Quality Control | Advanced quality control measures—including genetic stability assessments and pluripotency verification—are integrated throughout the expansion process. |
Coriell’s team of experts continually optimizes protocols to meet the growing demands of biomedical research and clinical applications, making us a trusted leader in iPSC expansion.
With continued innovation and the expertise of the Coriell Institute, iPSCs are poised to revolutionize medicine. From regenerative therapies and personalized drug testing to disease modeling, the ability to expand iPSCs efficiently and cost-effectively is central to their transformative potential in biomedical research and clinical applications.
