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Stem Cell Core Services
- Reprogramming
- Characterization
- Differentiated Cell Lines
- Expansion and Banking
- iPSC-derived Organoids
Key iPSC Quality Control Assays
- Post-thaw viability and growth rate
- Sterility
- Mycoplasma
- Identity match (DNA fingerprinting)
- G-banded karyotype
- Affymetrix Genome-Wide Human SNP Array 6.0
- Confirmation of integration-free reprogramming
- Pluripotency
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- Alkaline phosphatase staining (AP Assay)
- Immunocytochemistry (ICC) – cell surface markers
- Flow cytometry – cell surface markers
- hPSC Scorecard Assay
- Tri-lineage differentiation potential
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Biobanking
- Establishment of public and private repositories and independent biobanks
- Banking, cryopreservation and fail-safe storage of cell lines, DNA, and other biomaterials
- Repository Information Management Systems
- Online catalogs with annotation of samples
- Collection and distribution systems, online tracking of all shipments
- Controlled-access to samples and associated data
Research Support Services
- Primary and transformed cell lines
- DNA and RNA isolation and quality control
- Data analysis
- Designing a study that includes microarray
- Quality assurance certification
- Customized project reports
- Ensuring your study design accounts for participant confidentiality
- Advice on human subject research and Institutional Review Board approval
- Shipping of biospecimens domestically and internationally
- Controlled-access to samples and associated data
Collection Kits
- Standard kits
- Custom kits
Coriell Marketplace
- Online catalog
- Custom lab services
- Global distribution
Sample Procurement
- Infectious diseases
- Cancer
- Respiratory
- Cardiology
- Apparently healthy/controls
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Genomic and Epigenomic Services
Epigenetics
- Genome-wide DNA methylation analysis: reduced representation bisulfite sequencing (RRBS), whole genome bisulfite sequencing (WGBS), digital restriction enzyme analysis of methylation (DREAM).
- Gene-specific DNA methylation analysis: bisulfite pyrosequencing, bisulfite amplicon sequencing
- Chromatin accessibility analysis (ATAC-seq) for mapping of opened and closed chromatin
- Chromatin immunoprecipitation (ChIP-seq) for histone marks and DNA-binding proteins
Bioinformatic analyses of next generation sequencing data
- DNA: low pass or high coverage genomic sequencing, whole exome sequencing, custom targeted sequencing, amplicon sequencing
- RNA: polyA, random primed, strand-specific sequencing
Single cell RNA-sequencing
Nucleic Acid Isolation and Quality Control
- DNA and RNA Isolation
- Customized Product Preparations
- Identity Testing
- Species Identity Testing
- Sex Determination Assay
- Verification of Family Pedigrees
- Mycoplasma Screening
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Biomarkers
- Receipt and storage of a variety of samples at optimal temperature including blood, plasma, serum, CSF, urine, saliva, blood DNA and RNA
- Processing of samples to yield DNA, RNA, including rigorous QC
- Reporting of life-cycle of samples; enterprise level LIMS
- Hemoglobin assays to assess quality of serum, plasma, and cerebrospinal fluid
- Development of additional biomarker assays and Quality Control
- Controlled access to samples and associated data
Cell Culture
- Establishment of cell lines from tissue and blood
- Fibroblasts, endothelial, smooth muscle, and epithelial cell cultures from biopsies
- Epstein-Barr virus transformed lymphoblast lines
- Isolation and cryopreservation of buffy coats (peripheral blood mononuclear cells)
- Expansion of cell lines for distribution, stock maintenance, molecular and cytogenetic analysis
- Quality control assessment of existing cell lines
- Detection of mycoplasma contamination
- Fail-safe storage of plasma, serum, and other biosamples
- Controlled-access to samples and associated data
- Custom cell banking
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