Description:
HTT-Q23-FRT, MIX, 1-3144, HUMAN
HUNTINGTON DISEASE; HD
Repository
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HD Community Biorepository
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Quantity |
20 µg |
Quantitation Method |
Please see our FAQ |
Certain Third Party Licensing Requirements |
Invitrogen License for Cat# V800-20 |
Alias
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FLmixQ23f
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Sample Source
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CHDI Foundation, Inc.
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Family History
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N
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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Remarks |
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n
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Construct Name |
FLmixQ23f |
Insert Length |
9454 |
Vector |
pcDNA5/FRT |
Concentration |
64.1 ng/ml |
Sequence |
GATAAACACCACCATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAGT CCTTCCAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACAGCAGCAACAGCAA CAACAGCAGCAACAGCCGCCACCGCCGCCGCCGCCGCCGCCGCCTCCTCAGCTTCCTCA GCCGCCGCCGCAGGCACAGCCGCTGCTGCCTCAGCCGCAGCCGCCCCCGCCGCCGCCCC CGCCGCCACCCGGCCCGGCTGTGGCTGAGGAGCCGCTGCACCGACCAAAGAAAGAACTT TCAGCTACCAAGAAAGACCGTGTGAATCATTGTCTGACAATATGTGAAAACATAGTGGC ACAGTCTGTCAGAAATTCTCCAGAATTTCAGAAACTTCTGGGCATCGCTATGGAACTTT TTCTGCTGTGCAGTGATGACGCAGAGTCAGATGTCAGGATGGTGGCTGACGAATGCCTC AACAAAGTTATCAAAGCTTTGATGGATTCTAATCTTCCAAGGTTACAGCTCGAGCTCTA TAAGGAAATTAAAAAGAATGGTGCCCCTCGGAGTTTGCGTGCTGCCCTGTGGAGGTTTG CTGAGCTGGCTCACCTGGTTCGGCCTCAGAAATGCAGGCCTTACCTGGTGAACCTTCTG CCGTGCCTGACTCGAACAAGCAAGAGACCCGAAGAATCAGTCCAGGAGACCTTGGCTGC AGCTGTTCCCAAAATTATGGCTTCTTTTGGCAATTTTGCAAATGACAATGAAATTAAGG TTTTGTTAAAGGCCTTCATAGCGAACCTGAAGTCAAGCTCCCCCACCATTCGGCGGACA GCGGCTGGATCAGCAGTGAGCATCTGCCAGCACTCAAGAAGGACACAATATTTCTATAG TTGGCTACTAAATGTGCTCTTAGGCTTACTCGTTCCTGTCGAGGATGAACACTCCACTC TGCTGATTCTTGGCGTGCTGCTCACCCTGAGGTATTTGGTGCCCTTGCTGCAGCAGCAG GTCAAGGACACAAGCCTGAAAGGCAGCTTCGGAGTGACAAGGAAAGAAATGGAAGTCTC TCCTTCTGCAGAGCAGCTTGTCCAGGTTTATGAACTGACGTTACATCATACACAGCACC AAGACCACAATGTTGTGACCGGAGCCCTGGAGCTGTTGCAGCAGCTCTTCAGAACGCCT CCACCCGAGCTTCTGCAAACCCTGACCGCAGTCGGGGGCATTGGGCAGCTCACCGCTGC TAAGGAGGAGTCTGGTGGCCGAAGCCGTAGTGGGAGTATTGTGGAACTTATAGCTGGAG GGGGTTCCTCATGCAGCCCTGTCCTTTCAAGAAAACAAAAAGGCAAAGTGCTCTTAGGA GAAGAAGAAGCCTTGGAGGATGACTCTGAATCGAGATCGGATGTCAGCAGCTCTGCCTT AACAGCCTCAGTGAAGGATGAGATCAGTGGAGAGCTGGCTGCTTCTTCAGGGGTTTCCA CTCCAGGGTCAGCAGGTCATGACATCATCACAGAACAGCCACGGTCACAGCACACACTG CAGGCGGACTCAGTGGATCTGGCCAGCTGTGACTTGACAAGCTCTGCCACTGATGGGGA TGAGGAGGATATCTTGAGCCACAGCTCCAGCCAGGTCAGCGCCGTCCCATCTGACCCTG CCATGGACCTGAATGATGGGACCCAGGCCTCGTCGCCCATCAGCGACAGCTCCCAGACC ACCACCGAAGGGCCTGATTCAGCTGTTACCCCTTCAGACAGTTCTGAAATTGTGTTAGA CGGTACCGACAACCAGTATTTGGGCCTGCAGATTGGACAGCCCCAGGATGAAGATGAGG AAGCCACAGGTATTCTTCCTGATGAAGCCTCGGAGGCCTTCAGGAACTCTTCCATGGCC CTTCAACAGGCACATTTATTGAAAAACATGAGTCACTGCAGGCAGCCTTCTGACAGCAG TGTTGATAAATTTGTGTTGAGAGATGAAGCTACTGAACCGGGTGATCAAGAAAACAAGC CTTGCCGCATCAAAGGTGACATTGGACAGTCCACTGATGATGACTCTGCACCTCTTGTC CATTGTGTCCGCCTTTTATCTGCTTCGTTTTTGCTAACAGGGGGAAAAAATGTGCTGGT TCCGGACAGGGATGTGAGGGTCAGCGTGAAGGCCCTGGCCCTCAGCTGTGTGGGAGCAG CTGTGGCCCTCCACCCGGAATCTTTCTTCAGCAAACTCTATAAAGTTCCTCTTGACACC ACGGAATACCCTGAGGAACAGTATGTCTCAGACATCTTGAACTACATCGATCATGGAGA CCCACAGGTTCGAGGAGCCACTGCCATTCTCTGTGGGACCCTCATCTGCTCCATCCTCA GCAGGTCCCGCTTCCACGTGGGAGATTGGATGGGCACCATTAGAACCCTCACAGGAAAT ACATTTTCTTTGGCGGATTGCATTCCTTTGCTGCGGAAAACACTGAAGGATGAGTCTTC TGTTACTTGCAAGTTAGCTTGTACAGCTGTGAGGAACTGTGTCATGAGTCTCTGCAGCA GCAGCTACAGTGAGTTAGGACTGCAGCTGATCATCGATGTGCTGACTCTGAGGAACAGT TCCTATTGGCTGGTGAGGACAGAGCTTCTGGAAACCCTTGCAGAGATTGACTTCAGGCT GGTGAGCTTTTTGGAGGCAAAAGCAGAAAACTTACACAGAGGGGCTCATCATTATACAG GGCTTTTAAAACTGCAAGAACGAGTGCTCAATAATGTTGTCATCCATTTGCTTGGAGAT GAAGACCCCAGGGTGCGACATGTTGCCGCAGCATCACTAATTAGGCTTGTCCCAAAGCT GTTTTATAAATGTGACCAAGGACAAGCTGATCCAGTAGTGGCCGTGGCAAGAGATCAAA GCAGTGTTTACCTGAAACTTCTCATGCATGAAACGCAGCCTCCATCTCATTTCTCCGTC AGCACAATAACCAGAATATATAGAGGCTATAACCTACTACCAAGCATAACAGACGTCAC TATGGAAAATAACCTTTCAAGAGTTATTGCAGCAGTTTCTCATGAACTAATCACATCAA CCACCAGAGCACTCACATTTGGATGCTGTGAAGCTTTGTGTCTTCTTTCCACTGCCTTC CCAGTTTGCATTTGGAGTTTAGGTTGGCACTGTGGAGTGCCTCCACTGAGTGCCTCAGA TGAGTCTAGGAAGAGCTGTACCGTTGGGATGGCCACAATGATTCTGACCCTGCTCTCGT CAGCTTGGTTCCCATTGGATCTCTCAGCCCATCAAGATGCTTTGATTTTGGCCGGAAAC TTGCTTGCAGCCAGTGCTCCCAAATCTCTGAGAAGTTCATGGGCCTCTGAAGAAGAAGC CAACCCAGCAGCCACCAAGCAAGAGGAGGTCTGGCCAGCCCTGGGGGACCGGGCCCTGG TGCCCATGGTGGAGCAGCTCTTCTCTCACCTGCTGAAGGTGATTAACATTTGTGCCCAC GTCCTGGATGACGTGGCTCCTGGACCCGCAATAAAGGCAGCCTTGCCTTCTCTAACAAA CCCCCCTTCTCTAAGTCCCATCCGACGAAAGGGGAAGGAGAAAGAACCAGGAGAACAAG CATCTGTACCGTTGAGTCCCAAGAAAGGCAGTGAGGCCAGTGCAGCTTCTAGACAATCT GATACCTCAGGTCCTGTTACAACAAGTAAATCCTCATCACTGGGGAGTTTCTATCATCT TCCTTCATACCTCAAACTGCATGATGTCCTGAAAGCTACACACGCTAACTACAAGGTCA CGCTGGATCTTCAGAACAGCACGGAAAAGTTTGGAGGGTTTCTCCGCTCAGCCTTGGAT GTTCTTTCTCAGATACTAGAGCTGGCCACACTGCAGGACATTGGGAAGTGTGTTGAAGA GATCCTAGGATACCTGAAATCCTGCTTTAGTCGAGAACCAATGATGGCAACTGTTTGTG TTCAACAATTGTTGAAGACTCTCTTTGGCACAAACTTGGCCTCCCAGTTTGATGGCTTA TCTTCCAACCCCAGCAAGTCACAAGGCCGAGCACAGCGCCTTGGCTCCTCCAGTGTGAG GCCAGGCTTGTACCACTACTGCTTCATGGCCCCGTACACCCACTTCACCCAGGCCCTCG CTGACGCCAGCcTGAGGAACATGGTGCAGGCGGAGCAGGAGAACGACACCTCGGGATGG TTTGATGTCCTCCAGAAAGTGTCTACCCAGTTGAAGACAAACCTCACGAGTGTCACAAA GAACCGTGCAGATAAGAATGCTATTCATAATCACATTCGTTTGTTTGAACCTCTTGTTA TAAAAGCTTTAAAACAGTACACGACTACAACATGTGTGCAGTTACAGAAGCAGGTTTTA GATTTGCTGGCGCAGCTGGTTCAGTTACGGGTTAATTACTGTCTTCTGGATTCAGATCA GGTGTTTATTGGCTTTGTATTGAAACAGTTTGAATACATTGAAGTGGGCCAGTTCAGGG AATCAGAGGCAATCATTCCAAACATCTTTTTCTTCTTGGTATTACTATCTTATGAACGC TATCATTCAAAACAGATCATTGGAATTCCTAAAATCATTCAGCTCTGTGATGGCATCAT GGCCAGTGGAAGGAAGGCTGTGACACATGCCATACCGGCTCTGCAGCCCATAGTCCACG ACCTCTTTGTATTAAGAGGAACAAATAAAGCTGATGCAGGAAAAGAGCTTGAAACCCAA AAAGAGGTGGTGGTGTCAATGTTACTGAGACTCATCCAGTACCATCAGGTGTTGGAGAT GTTCATTCTTGTCCTGCAGCAGTGCCACAAGGAGAATGAAGACAAGTGGAAGCGACTGT CTCGACAGATAGCTGACATCATCCTCCCAATGTTAGCCAAACAGCAGATGCACATTGAC TCTCATGAAGCCCTTGGAGTGTTAAATACATTATTTGAGATTTTGGCCCCTTCCTCCCT CCGTCCGGTAGACATGCTTTTACGGAGTATGTTCGTCACTCCAAACACAATGGCGTCCG TGAGCACTGTTCAACTGTGGATATCGGGAATTCTGGCCATTTTGAGGGTTCTGATTTCC CAGTCAACTGAAGATATTGTTCTTTCTCGTATTCAGGAGCTCTCCTTCTCTCCGTATTT AATCTCCTGTACAGTAATTAATAGGTTAAGAGATGGGGACAGTACTTCAACGCTAGAAG AACACAGTGAAGGGAAACAAaTAAAGAATTTGCCAGAAgAAACATTTTCAAGGTTTCTA TTACAACTGGTTGGTATTCTTTTAGAAGACATTGTTACAAAACAGCTGAAGGTGGAAAT GAGTGAGCAGCAACATACTTTCTATTGCCAGGAACTAGGCACACTGCTAATGTGTCTGA TCCACATCTTCAAGTCTGGAATGTTCCGGAGAATCACAGCAGCTGCCACTAGGCTGTTC CGCAGTGATGGCTGTGGCGGCAGTTTCTACACCCTGGACAGCTTGAACTTGCGGGCTCG TTCCATGATCACCACCCACCCGGCCCTGGTGCTGCTCTGGTGTCAGATACTGCTGCTTG TCAACCACACCGACTACCGCTGGTGGGCAGAAGTGCAGCAGACCCCGAAAAGACACAGT CTGTCCAGCACAAAGTTACTTAGTCCCCAGATGTCTGGAGAAGAGGAGGATTCTGACTT GGCAGCCAAACTTGGAATGTGCAATAGAGAAATAGTACGAAGAGGGGCTCTCATTCTCT TCTGTGATTAAGTCTGTCAGAACCTCCATGACTCCGAGCACTTAACGTGGCTCATTGTA AATCACATTCAAGATCTGATCAGCCTTTCCCACGAGCCTCCAGTACAGGACTTCATCAG TGCCGTTCATCGGAACTCTGCTGCCAGCGGCCTGTTCATCCAGGCAATTCAGTCTCGTT GTGAAAACCTTTCAACTCCAACCATGCTGAAGAAAACTCTTCAGTGCTTGGAGGGGATC CATCTCAGCCAGTCGGGAGCTGTGCTCACGCTGTATGTGGACAGGCTTCTGTGCACCCC TTTCCGTGTGCTGGCTCGCATGGTCGACATCCTTGCTTGTCGCCGGGTAGAAATGCTTC TGGCTGCAAATTTACAGAGCAGCATGGCCCAGTTGCCAATGGAAGAACTCAACAGAATC CAGGAATACCTTCAGAGCAGCGGGCTCGCTCAGAGACACCAAAGGCTCTATTCCCTGCT GGACAGGTTTCGTCTGTCCACCATGCAAGACTCACTTAGTCCCTCTCCTCCAGTCTCTT CCCACCCGCTGGACGGGGATGGGCACGTGTCACTGGAAACAGTGAGTCCGGACAAAGAC TGGTACGTTCATCTTGTCAAATCCCAGTGTTGGACCAGGTCAGATTCTGCACTGCTGGA AGGTGCAGAGCTGGTGAATCGGATTCCTGCTGAAGATATGAATGCCTTCATGATGAACT CGGAGTTCAACCTAAGCCTGCTAGCTCCATGCTTAAGCCTAGGGATGAGTGAAATTTCT GGTGGCCAGAAGAGTGCCCTTTTTgAAGCAGCCCGTGAGGTGACTCTGGCCCGTGTGAG CGGCACCGTGCAGCAGCTCCCTGCTGTCCATCATGTCTTCCAGCCCGAGCTGCCTGCAG AGCCGGCGGCCTACTGGAGCAAGTTGAATGATCTGTTTGGGGATGCTGCACTGTATCAG TCCCTGCCCACTCTGGCCCGGGCCCTGGCACAGTACCTGGTGGTGGTCTCCaAACTGCC CAGTCATTTGCACCTTCCTCCTGAGAAAGAGAAGGACATTGTGAAATTCGTGGTGGCAA CCCTTGAGGCCCTGTCCTGGCATTTGATCCATGAGCAGATCCCGCTGAGTCTGGATCTC CAGGCAGGGCTGGACTGCTGCTGCCTGGCCCTGCAGCTGCCTGGCCTCTGGAGCGTGGT CTCCTCCACAGAGTTTGTGACCCACGCCTGCTCCCTCATCTACTGTGTGCACTTCATCC TGGAGGCCGTTGCAGTGCAGCCTGGAGAGCAGCTTCTTAGTCCAGAAAGAAGGACAAAT ACCCCAAAAGCCATCAGCGAGGAGGAGGAGGAAGTAGATCCAAACACACAGAATCCTAA GTATATCACTGCAGCCTGTGAGATGGTGGCAGAAATGGTGGAGTCTCTGCAGTCGGTGT TGGCCTTGGGTCATAAAAGGAATAGCGGCGTGCCGGCGTTTCTCACGCCATTGCTCAGG AACATCATCATCAGCCTGGCCCGCCTGCCCCTTGTCAACAGCTACACACGTGTGCCCCC ACTGGTGTGGAAGCTTGGATGGTCACCCAAACCGGGAGGGGATTTTGGCACAGCATTCC CTGAGATCCCCGTGGAGTTCCTCCAGGAAAAGGAAGTCTTTAAGGAGTTCATCTACCGC ATCAACACACTAGGCTGGACCAGTCGTACTCAGTTTGAAGAAACTTGGGCCACCCTCCT TGGTGTCCTGGTGACGCAGCCCCTCGTGATGGAGCAGGAGGAGAGCCCACCAGAAGAAG ACACAGAGAGGACCCAGATCAACGTCCTGGCCGTGCAGGCCATCACCTCACTGGTGCTC AGTGCAATGACTGTGCCTGTGGCCGGCAACCCAGCTGTAAGCTGCTTGGAGCAGCAGCC CCGGAACAAGCCTCTGAAAGCTCTCGACACCAGGTTTGGGAGGAAGCTGAGCATTATCA GAGGGATTGTGGAGCAAGAGATTCAAGCAATGGTTTCAAAGAGAGAGAATATTGCCACC CATCATTTATATCAGGCATGGGATCCTGTCCCTTCTCTGTCTCCGGCTACTACAGGTGC CCTCATCAGCCACGAGAAGCTGCTGCTACAGATCAACCCCGAGCGGGAGCTGGGGAGCA TGAGCTACAAACTCGGCCAGGTGTCCATACACTCCGTGTGGCTGGGGAACAGCATCACA CCCCTGAGGGAGGAGGAATGGGACGAGGAAGAGGAGGAGGAGGCCGACGCCCCTGCACC TTCGTCACCACCCACTTCTCCAGTCAACTCCAGGAAACACCGGGCTGGAGTTGACATCC ACTCCTGTTCGCAGTTTTTGCTTGAGTTGTACAGCCGCTGGATCCTGCCGTCCAGCTCA GCCAGGAGGACCCCGGCCATCCTGATCAGTGAGGTGGTCAGATCCCTTCTAGTGGTCTC AGACTTGTTCACCGAGCGCAACCAGTTTGAGCTGATGTATGTGACGCTGACAGAACTGC GAAGGGTGCACCCTTCAGAAGACGAGATCCTCGCTCAGTACCTGGTGCCTGCCACCTGC AAGGCAGCTGCCGTCCTTGGGATGGACAAGGCCGTGGCGGAGCCTGTCAGCCGCCTGCT GGAGAGCACGCTCAGGAGCAGCCACCTGCCCAGCAGGGTTGGAGCCCTGCACGGCGTCC TCTATGTGCTGGAGTGCGACCTGCTGGACGACACTGCCAAGCAGCTCATCCCGGTCATC AGCGACTATCTCCTCTCCAACCTGAAAGGGATCGCCCACTGCGTGAACATTCACAGCCA GCAGCACGTACTGGTCATGTGTGCCACTGCGTTTTACCTCATTGAGAACTATCCTCTGG ACGTAGGGCCGGAATTTTCAGCATCAATAATACAGATGTGTGGGGTGATGCTGTCTGGA AGTGAGGAGTCCACCCCCTCCATCATTTACCACTGTGCCCTCAGAGGCCTGGAGCGCCT CCTGCTCTCTGAGCAGCTCTCCCGCCTGGATGCAGAATCGCTGGTCAAGCTGAGTGTGG ACAGAGTGAACGTGCACAGCCCGCACCGGGCCATGGCGGCTCTGGGCCTGATGCTCACC TGCATGTACACAGGAAAGGAGAAAGTCAGTCCGGGTAGAACTTCAGACCCTAATCCTGC AGCCCCCGACAGCGAGTCAGTGATTGTTGCTATGGAGCGGGTATCTGTTCTTTTTGATA GGATCAGGAAAGGCTTTCCTTGTGAAGCCAGAGTGGTGGCCAGGATCCTGCCCCAGTTT CTAGACGACTTCTTCCCACCCCAGGACATCATGAACAAAGTCATCGGAGAGTTTCTGTC CAACCAGCAGCCATACCCCCAGTTCATGGCCACCGTGGTGTATAAGGTGTTTCAGACTC TGCACAGCACCGGGCAGTCGTCCATGGTCCGGGACTGGGTCATGCTGTCCCTCTCCAAC TTCACGCAGAGGGCCCCGGTCGCCATGGCCACGTGGAGCCTCTCCTGCTTCTTTGTCAG CGCGTCCACCAGCCCGTGGGTCGCGGCGATCCTCCCACATGTCATCAGCAGGATGGGCA AGCTGGAGCAGGTGGACGTGAACCTTTTCTGCCTGGTCGCCACAGACTTCTACAGACAC CAGATAGAGGAGGAGCTCGACCGCAGGGCCTTCCAGTCTGTGCTTGAGGTGGTTGCAGC CCCAGGAAGCCCATATCACCGGCTGCTGACTTGTTTACGAAATGTCCACAAGGTCACCA CCTGCGCGGCCGC
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Cloning Information |
The construct is engineered with a "PmeI" on the 5' and NotI on the 3' prime. Cloning site: fused EcoRV/PmeI at 5' and Not I at 3', RV and PmeI were deleted. |
Remarks |
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n
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DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n
|
Remarks |
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a mixed codon. mix= [CAG, CAA, CAG, CAA, CAA]n
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PDF |
Vector Map |
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Plasmid Collection Screening and Propagation |
Supplement |
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