GM03716
LCL from B-Lymphocyte
Description:
TRISOMY 21
ANEUPLOID CHROMOSOME NUMBER - TRISOMY
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Heritable Diseases Chromosome Abnormalities |
Class |
Other Disorders of Known Biochemistry |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
47,XY,+21[42]/47,XY,add(9)(?::p24>qter),+21[8]]
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Cytogenetics |
Chromosome 21: ANEUPLOID Aneuploid Segment (+)21pter>21qter |
|
Chromosome 21: ANEUPLOID Trisomic Segment 21pter>21qter |
Remarks |
Clinical features of Down syndrome |
Lemieux N, Malfoy B, Forrest GL, Human carbonyl reductase (CBR) localized to band 21q22.1 by high- resolution fluorescence in situ hybridization displays gene dosage effects in trisomy 21 cells. Genomics15:169-72 1993 |
PubMed ID: 8432528 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
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