Culture Medium Information

For reference see: Morton, H.C., A survey of Commercially Available Tissue Culture Media. In Vitro 6: 89-108 (1970).

The code consists of an abbreviation of the culture media followed by a number, indicating the percentage of fetal bovine serum, and the letter "I" or "U". "I" indicates that the fetal bovine serum is heat inactivated at 56°C for 1/2 hour before it is added to the culture medium. "U" indicates that the fetal bovine serum is used without prior inactivation (not inactivated). The abbreviations are as follows:

MEDIUM

DESCRIPTION

AFCM

Amniotic Fluid Culture Medium

AMDUM

Equal volumes of Alpha MEM (Eagle) with nucleosides and Dulbecco’s Modified Eagles Medium

AMEM

MEM (Eagle), Alpha Modification with nucleosides

AMMC

Equal volumes of Alpha MEM (Eagle) and MCDB 110 Medium

ASCM

Adipose Stromal Cell Medium

CnT-07

CnT-07 Epidermal Progenitor Cell Medium

DKSFM

Defined Keratinocyte Serum-Free Medium

DUF12

Equal volumes of Dulbecco’s Modified Eagles Medium and Ham’s F-12 Nutrient Mix

DUMEM

Dulbecco’s Modified Eagles Medium (high glucose-4.5 g/L)

EEDUM

Equal volumes Minimum Essential Medium (Eagle) in Earle’s BSS with essential and nonessential amino acids and vitamins and Dulbecco’s Modified Eagles Medium (high glucose-4.5 g/L)

EEMEM

Minimum Essential Medium (Eagle) in Earle’s BSS with essential and nonessential amino acids and vitamins

EEMMC

Equal volumes of Minimum Essential Medium (Eagle) in Earle’s BSS with essential and nonessential amino acids and vitamins and MCDB 110 Medium

HEMEM

Minimum Essential Medium (Eagle) in Hank’s BSS, plus nonessential amino acids and vitamins

HF10

Ham’s F-10 Nutrient Mix

HF12

Ham’s F-12 Nutrient Mix

KGM

Keratinocyte Growth Medium

M153LEIB

Four parts MCDB 153 Medium and 1 part Leibovitz L-15 Medium

M199

Medium 199 in Earle BSS

MEGM

Mammary Epithelial Cell Medium plus the following supplements: insulin (5 µg/ml), hydrocortisone (0.5 µg/ml), Epidermal Growth Factor (EGF, 5ng/ml) and Bovine Pituitary Extract (70 µg/ml)

MES

Equal volumes of Medium 199 in Earle’s BSS and MCDB 110 Medium

MGM

MGM-4 Melanocyte Growth Medium-4

R1640

Roswell Park Memorial Institute Medium 1640

SMEM

Minimum Essential Medium Modified for Suspension Cultures