GM05384
Tumor-derived cell line from Adrenal cortex tumor
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Adrenal cortex tumor
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Tumor-derived cell line from Adrenal cortex tumor
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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40,XY,1q+
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| Passage Frozen |
48 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Y-1; epithelial-like cell culture established from an adrenal cortex tumor of a male LAF1 mouse; secretes steroid hormones; reverse transcriptase positive; 40,XY,1q+; 40% of cells have 1 or 2 centric fragments |
| George DL, Powers VE, Amplified DNA sequences in Y1 mouse adrenal tumor cells: association with double minutes and localization to a homogeneously staining chromosomal region. Proc Natl Acad Sci U S A79:1597-601 1982 |
| PubMed ID: 6951198 |
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| George DL, Francke U, Homogeneously staining chromosome regions and double minutes in a mouse adrenocortical tumor cell line. Cytogenet Cell Genet28:217-26 1980 |
| PubMed ID: 6936133 |
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| Cohen, In vitro response of functional experimental adrenal tumors to corticotropin (ACTH). Proc Soc Exp Biol Med95:304 (1975):217-26 1975 |
| PubMed ID: 6936133 |
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| Yasumura Y, Buonassisi V, Sato G, Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res26:529-35 1966 |
| PubMed ID: 5930699 |
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| Buonassisi, Hormone-producing cultures of adrenal and pituitary tumor origin. Proc Natl Acad Sci USA48:1184 (1962):529-35 1962 |
| PubMed ID: 5930699 |
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| Cohen, Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am J Pathol33:631 (1957):529-35 1957 |
| PubMed ID: 5930699 |
| Passage Frozen |
48 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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