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GM05862 Fibroblast from Skin, Embryo

Description:

MUS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Animals
Biopsy Source Embryo
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Embryo
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 73~78,X~XXYYYY,-1,-4,+6,-7,+8,+8,-9,-10,+11,+der(11;17)(A1;A1),-12,del(12)(D3),-13,-14,+15,-16,-17,-18,-19,+2~7mar[cp10]
Species Mus musculus
Common Name house mouse
Remarks Mouse line 3T6; fibroblast cell line established by Todaro and Green from Swiss albino mouse embryos; secretes collagen and hyaluronic acid

Characterizations

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Passage Frozen 38
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Genbank COX1

Phenotypic Data

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Remarks Mouse line 3T6; fibroblast cell line established by Todaro and Green from Swiss albino mouse embryos; secretes collagen and hyaluronic acid

Publications

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Shackley DC, Haylett A, Whitehurst C, Betts CD, O'Flynn K, Clarke NW, Moore JV, Comparison of the cellular molecular stress responses after treatments used in bladder cancer. BJU Int90(9):924-32 2002
PubMed ID: 12460358
 
Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996
PubMed ID: 8661066
 
Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995
PubMed ID: 7860074
 
Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993
PubMed ID: 8478002
 
Draber P, Stanley P, Isolation and partial characterization of lectin-resistant F9 cells. Somat Cell Mol Genet10:445-54 1984
PubMed ID: 6591444
 
Buck DW, Kennett RH, McGarrity G, Monoclonal antibodies specific for cell culture mycoplasmas. In Vitro18:377-81 1982
PubMed ID: 6811415
 
Green H, Goldberg B, Todaro GJ, Differentiated cell types and the regulation of collagen synthesis. Nature212:631-3 1966
PubMed ID: 5971697
 
Todaro, Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol17:299 (1963):631-3 1963
PubMed ID: 5971697

External Links

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dbSNP dbSNP ID: 10838

Culture Protocols

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Passage Frozen 38
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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