GM10478

GM10478 Somatic cell hybrid

Description:

ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Hybrids

Cell Type

Somatic cell hybrid

Transformant

Untransformed

Family Member

Confirmation

Karyotypic analysis and Southern blot hybridization

ISCN

Mouse/human somatic cell hybrid retaining human chromosome #20 in 60% of cells & #4 in 4% of cells

Remarks

Line HDm-4; produced by microcell-mediated chromo transfer of hum foreskin fibro chromos with retroviral vector insertions & 3T6 mouse cells; selected in medium with G418 (0.25 mg/ml); retains hum chroms #20 (56% of cells) & #4 (18%)

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis

GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, and 4q11-q13, AFP.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 7p12, EGFR, and gave a negative result with a primer for 7q31.3, CFTR.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8

DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 8p21, NEFL. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p21, D9S270, and 9pter-q12, RLN1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10

DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 10p15p14, IL2R, and 10q24qter, PLAU. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10p11.2, ITGB1, and gave a negative result with a primer for 10q24, PLAU.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11q23.3, ETS1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15

DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q11.2-q12, GABRB3.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20

DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 20p12, D20S6, and 20q13.2, D20S4. Amplification with PCR primers D20S100 and D20S93, specific for chromosome 20, failed to show a positive signal (T. Lewis, San Antonio, TX, personal communication) Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2.

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22

Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 22q11.2, IGLC2.

Phenotypic Data

Remarks

Publications

Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998

PubMed ID: 9479491

Katsanis N, Fisher EM, A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3. Genomics47:294-9 1998

PubMed ID: 9479502

Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997

PubMed ID: 9070934

McCusker D, Jones T, Sheer D, Trowsdale J, Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex. Genomics45:362-7 1997

PubMed ID: 9344661

Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997

PubMed ID: 9207223

Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995

PubMed ID: 7601467

Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995

PubMed ID: 8530103

Kelsell DP, Rooke L, Warne D, Bouzyk M, Cullin L, Cox S, West L, Povey S, Spurr NK, Development of a panel of monochromosomal somatic cell hybrids for rapid gene mapping Annals of human genetics59:233-41 1995

PubMed ID: 7625768

Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995

PubMed ID: 8666384

Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995

PubMed ID: 7774910

Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994

PubMed ID: 8196634

De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994

PubMed ID: 7927540

Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994

PubMed ID: 8139910

Loftus SK, Shiang R, Warrington JA, Bengtsson U, McPherson JD, Wasmuth JJ, Genes encoding adrenergic receptors are not clustered on the long arm of human chromosome 5. Cytogenet Cell Genet67:69-74 1994

PubMed ID: 8039425

Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994

PubMed ID: 7520568

Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994

PubMed ID: 8275700

Clark CC, Cohen I, Eichstetter I, Cannizzaro LA, McPherson JD, Wasmuth JJ, Iozzo RV, Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. Genomics18:249-60 1993

PubMed ID: 8288227

Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993

PubMed ID: 8314568

Figlewicz DA, Delattre O, Guellaen G, Krizus A, Thomas G, Zucman J, Rouleau GA, Mapping of human gamma-glutamyl transpeptidase genes on chromosome 22 and other human autosomes. Genomics17:299-305 1993

PubMed ID: 8104871

Maraia RJ, Driscoll CT, Bilyeu T, Hsu K, Darlington GJ, Multiple dispersed loci produce small cytoplasmic Alu RNA. Mol Cell Biol13:4233-41 1993

PubMed ID: 7686619

Rao PN, Hayworth R, Akots G, Pettenati MJ, Bowden DW, Physical localization of chromosome 20 markers using somatic cell hybrid cell lines and fluorescence in situ hybridization. Genomics14:532-5 1992

PubMed ID: 1427875

Lugo TG, Handelin B, Killary AM, Housman DE, Fournier RE, Isolation of microcell hybrid clones containing retroviral vector insertions into specific human chromosomes. Mol Cell Biol7:2814-20 1987

PubMed ID: 2823107

External Links

dbSNP

dbSNP ID: 11381

Culture Protocols

Split Ratio

1:25

Temperature

37 C

Percent CO2

Medium

Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent

Serum

10% fetal bovine serum Not inactivated

Substrate

None specified

Subcultivation Method

trypsin-EDTA

Supplement

Pricing

Commercial/For-profit:

$311.00USD

Academic/Non-profit/Government:

$176.00USD