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GM10567 Somatic cell hybrid

Description:

NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
Confirmation Clinical summary/Case history
ISCN Mouse/human somatic cell hybrid retaining human chromosome #16
Remarks Line CY18; produced by PEG fusion of human fibroblast (GM02860) with a 46,XY,t(3;16) (q13.2;q13) karyo and mouse cell line A9, HPRT- and APRT-; grown in medium with AAT and L-proline; 80% of cells retain an intact human chromosome #16

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xp22.2, DXS16; Xp22.32 (also Yp11.3), MIC2; and Xq28, DXS15. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD.
 
GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6pterp24, D6S7. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 8p21, NEFL. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 9p22, IFNB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, 9p21, D9S270, 9p12, 1F6, 9pter-p22, D9S178, and 9pter-q12, RLN1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 14q32.33, IGHG4, and 14q32.1q32.3, D14S13. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 16q22.1, HPR, and 16q22.1, LCAT, and gave a negative result with a primer for 16p13.3, HBA. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 16p13.3, HBA, 16q22.1, LCAT, and 16q24.2, D16S389.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 17p13.1, MYH2, and 17q21.3q23, MPO. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q23, MBP. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 19q13.3, LHB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2.
 

Phenotypic Data

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Remarks Line CY18; produced by PEG fusion of human fibroblast (GM02860) with a 46,XY,t(3;16) (q13.2;q13) karyo and mouse cell line A9, HPRT- and APRT-; grown in medium with AAT and L-proline; 80% of cells retain an intact human chromosome #16

Publications

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Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006
PubMed ID: 16537608
 
Krona C, Ejeskär K, Carén H, Abel F, Sjöberg RM, Martinsson T, A novel 1p362 located gene, APITD1, with tumour-suppressive properties and a putative p53-binding domain, shows low expression in neuroblastoma tumours British journal of cancer91:1119-30 2004
PubMed ID: 15328517
 
Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B, The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology Proceedings of the National Academy of Sciences of the United States of America101:1731-6 2004
PubMed ID: 14757826
 
Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ, Identification and characterization of human taste receptor genes belonging to the TAS2R family Cytogenetic and genome research98:45-53 2003
PubMed ID: 12584440
 
Hansford LM, Smith SA, Haber M, Norris MD, Cheung B, Marshall GM, Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2) Cytogenetic and genome research101:17-23 2003
PubMed ID: 14571131
 
Janssens B, Mohapatra B, Vatta M, Goossens S, Vanpoucke G, Kools P, Montoye T, van Hengel J, Bowles NE, van Roy F, Towbin JA, Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy Human genetics112:227-36 2003
PubMed ID: 12596047
 
Tarafa G, Prat E, Risques RA, González S, Camps J, Grau M, Guinó E, Moreno V, Esteller M, Herman JG, Germà JR, Miró R, Peinado MA, Capellá G, Common genetic evolutionary pathways in familial adenomatous polyposis tumors Cancer research63:5731-7 2003
PubMed ID: 14522893
 
Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998
PubMed ID: 9479491
 
Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997
PubMed ID: 9070934
 
Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997
PubMed ID: 9207223
 
Vermeesch JR, Petit P, Kermouni A, Renauld JC, Van Den Berghe H, Marynen P, The IL-9 receptor gene, located in the Xq/Yq pseudoautosomal region, has an autosomal origin, escapes X inactivation and is expressed from the Y. Hum Mol Genet6:1-8 1997
PubMed ID: 9002663
 
Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996
PubMed ID: 8661066
 
Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995
PubMed ID: 7601467
 
Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995
PubMed ID: 8530103
 
Bloch KD, Wolfram JR, Brown DM, Roberts JD Jr, Zapol DG, Lepore JJ, Filippov G, Thomas JE, Jacob HJ, Bloch DB, Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17. Genomics27:526-30 1995
PubMed ID: 7558036
 
Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995
PubMed ID: 8666384
 
Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994
PubMed ID: 8196634
 
De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994
PubMed ID: 7927540
 
Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994
PubMed ID: 8139910
 
Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994
PubMed ID: 7959758
 
Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994
PubMed ID: 7520568
 
Nagaoka H, Ozawa K, Matsuda F, Hayashida H, Matsumura R, Haino M, Shin EK, Fukita Y, Imai T, Anand R, et al, Recent translocation of variable and diversity segments of the human immunoglobulin heavy chain from chromosome 14 to chromosomes 15 and 16. Genomics22:189-97 1994
PubMed ID: 7959766
 
Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994
PubMed ID: 8275700
 
Barnes T, Parry P, Hart I, Jones C, Minet M, Patterson D, Regional mapping of the gene encoding dihydroorotate dehydrogenase, an enzyme involved in UMP synthesis, electron transport, and superoxide generation, to human chromosome region 16q22. Somat Cell Mol Genet19:405-11 1993
PubMed ID: 8211381
 
Chong SS, Kristjansson K, Zoghbi HY, Hughes MR, Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23. Genomics18:355-359 1993
PubMed ID: 8288239
 
Clark CC, Cohen I, Eichstetter I, Cannizzaro LA, McPherson JD, Wasmuth JJ, Iozzo RV, Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. Genomics18:249-60 1993
PubMed ID: 8288227
 
Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993
PubMed ID: 8314568
 
Figlewicz DA, Delattre O, Guellaen G, Krizus A, Thomas G, Zucman J, Rouleau GA, Mapping of human gamma-glutamyl transpeptidase genes on chromosome 22 and other human autosomes. Genomics17:299-305 1993
PubMed ID: 8104871
 
Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993
PubMed ID: 8478002
 
Maraia RJ, Driscoll CT, Bilyeu T, Hsu K, Darlington GJ, Multiple dispersed loci produce small cytoplasmic Alu RNA. Mol Cell Biol13:4233-41 1993
PubMed ID: 7686619
 
Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993
PubMed ID: 8307557
 
Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992
PubMed ID: 1505951
 
Hendricks-Taylor LR, Bachinski LL, Siciliano MJ, Fertitta A, Trask B, de Jong PJ, Ledbetter DH, Darlington GJ, The CCAAT/enhancer binding protein (C/EBP alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP beta) gene (CEBPB) maps to human chromosome 20q13.1. Genomics14:12-7 1992
PubMed ID: 1427819
 
Troelstra C, Landsvater RM, Wiegant J, van der Ploeg M, Viel G, Buys CH, Hoeijmakers JH, Localization of the nucleotide excision repair gene ERCC6 to human chromosome 10q11-q21. Genomics12:745-9 1992
PubMed ID: 1349298
 
Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990
PubMed ID: 2095701
 
Ledbetter SA, Garcia-Heras J, Ledbetter DH, "PCR-karyotype" of human chromosomes in somatic cell hybrids. Genomics8:614-22 1990
PubMed ID: 2276735
 
Callen DF, Hyland VJ, Baker EG, Fratini A, Simmers RN, Mulley JC, Sutherland GR, Fine mapping of gene probes and anonymous DNA fragments to the long arm of chromosome 16. Genomics2:144-53 1988
PubMed ID: 2900808
 
Callen DF, A mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet29:235-9 1986
PubMed ID: 3495225

External Links

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dbSNP dbSNP ID: 11397

Culture Protocols

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Split Ratio 1:5
Temperature 37 C
Percent CO2 8%
Medium Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement Adenine 7.5 x 10-5M
Supplement Aminopterin 1.6 x 10-6M
Supplement Proline 20ug/ml
Supplement Thymidine 3.2 x 10-5M
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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