GM10658
Fibroblast from Skin, Unspecified
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a negative result with a primer for Yq11, DYS227. |
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| Remarks |
Chinese hamster line RJK88; HPRT deficient; total number of chromosomes per cell ranged from 17 to 21; fibroblast culture |
| Delbridge ML, Lingenfelter PA, Disteche CM, Graves JA, The candidate spermatogenesis gene RBMY has a homologue on the human X
chromosome. Nat Genet22(3):223-4 1999 |
| PubMed ID: 10391206 |
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| Kogi M, Fukushige S, Lefevre C, Hadano S, Ikeda JE, A novel tandem repeat sequence located on human chromosome 4p: isolation and characterization. Genomics42:278-83 1997 |
| PubMed ID: 9192848 |
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| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995 |
| PubMed ID: 7860074 |
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| Ishida Y, Hadano S, Nagayama T, Tomiyasu H, Wakasa K, Ikeda J, Isolation and characterization of 21 novel expressed DNA sequences from the distal region of human chromosome 4p. Genomics22:302-12 1994 |
| PubMed ID: 7545969 |
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| Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994 |
| PubMed ID: 7520568 |
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| Yokoi H, Hadano S, Kogi M, Kang X, Wakasa K, Ikeda JE, Isolation of expressed sequences encoded by the human Xq terminal portion using microclone probes generated by laser microdissection. Genomics20:404-11 1994 |
| PubMed ID: 8034313 |
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| Dittrich B, Buiting K, Gross S, Horsthemke B, Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum Mol Genet2:1995-9 1993 |
| PubMed ID: 8111366 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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