GM14396
LCL from B-Lymphocyte
Description:
RING CHROMOSOME
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Ethnicity
|
HISPANIC
|
Family Member
|
2
|
Relation to Proband
|
twin sister
|
Confirmation
|
Karyotypic analysis before cell line submission to CCR
|
ISCN
|
48,XX,+r(1),+r(16)[40]/47,XX,+r(1)[14]/47,XX,+r(16)[34]/46,XX[12].ish r(1) (D1Z5+),r(16)(D16Z2+)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Remarks |
46,XX{12}/47,XX,+r(1){14}/47,XX,+r(16) {34}/48,XX,+r(1),+r(16){40}.ish r(1) (D1Z5+),+r(16).ish r(16)(D16Z2+) |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
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