AG06814-M
Fibroblast from Lung, Lung
Description:
WI-38 - NORMAL HUMAN FETAL LUNG FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Family Member
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2
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
34 |
| Passage Frozen |
26 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Rossi M, Anerillas C, Idda ML, Munk R, Shin CH, Donega S, Tsitsipatis D, Herman AB, Martindale JL, Yang X, Piao Y, Mazan-Mamczarz K, Fan J, Ferrucci L, Johnson PF, De S, Abdelmohsen K, Gorospe M, Pleiotropic effects of BAFF on the senescence-associated secretome and growth arrest eLife12: 2022 |
| PubMed ID: 37083495 |
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| Bauwens E, Parée T, Meurant S, Bouriez I, Hannart C, Wéra AC, Khelfi A, Fattaccioli A, Burteau S, Demazy C, Fransolet M, De Schutter C, Martin N, Théry J, Decanter G, Penel N, Bury M, Pluquet O, Garmyn M, Debacq-Chainiaux F, Senescence Induced by UVB in Keratinocytes Impairs Amino Acids Balance The Journal of investigative dermatology143:554-565.e9 2021 |
| PubMed ID: 36528129 |
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| Nassour J, Aguiar LG, Correia A, Schmidt TT, Mainz L, Przetocka S, Haggblom C, Tadepalle N, Williams A, Shokhirev MN, Akincilar SC, Tergaonkar V, Shadel GS, Karlseder J, Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis Nature614:767-773 2021 |
| PubMed ID: 36755096 |
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| Ni K, Ren J, Xu X, He Y, Finney R, Braun SMG, Hathaway NA, Crabtree GR, Muegge K, LSH mediates gene repression through macroH2A deposition Nature communications11:5647 2020 |
| PubMed ID: 33159050 |
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| Soifer I, Fong NL, Yi N, Ireland AT, Lam I, Sooknah M, Paw JS, Peluso P, Concepcion GT, Rank D, Hastie AR, Jojic V, Ruby JG, Botstein D, Roy MA, Fully Phased Sequence of a Diploid Human Genome Determined G3 (Bethesda, Md)11:5647 2020 |
| PubMed ID: 32631951 |
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| Johnstone SE, Reyes A, Qi Y, Adriaens C, Hegazi E, Pelka K, Chen JH, Zou LS, Drier Y, Hecht V, Shoresh N, Selig MK, Lareau CA, Iyer S, Nguyen SC, Joyce EF, Hacohen N, Irizarry RA, Zhang B, Aryee MJ, Bernstein BE, Large-Scale Topological Changes Restrain Malignant Progression in Colorectal Cancer Cell182:1474-1489.e23 2019 |
| PubMed ID: 32841603 |
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| Perelygina L, Chen MH, Suppiah S, Adebayo A, Abernathy E, Dorsey M, Bercovitch L, Paris K, White KP, Krol A, Dhossche J, Torshin IY, Saini N, Klimczak LJ, Gordenin DA, Zharkikh A, Plotkin S, Sullivan KE, Icenogle J, Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies PLoS pathogens15:e1008080 2019 |
| PubMed ID: 31658304 |
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| Horowitz B1, Javitt G1, Ilani T1, Gat Y1, Morgenstern D2, Bard FA3, Fass D, Quiescin sulfhydryl oxidase 1 (QSOX1) glycosite mutation perturbs secretion but not Golgi localization Glycobiology28:580-591 2018 |
| PubMed ID: 29757379 |
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| Nassour J, Radford R, Correia A, Fusté JM, Schoell B, Jauch A, Shaw RJ, Karlseder J, Autophagic cell death restricts chromosomal instability during replicative crisis Nature565:659-663 2018 |
| PubMed ID: 30675059 |
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| Anderton RS, Meloni BP, Mastaglia FL, Greene WK, Boulos S, Survival of motor neuron protein over-expression prevents calpain-mediated cleavage and activation of procaspase-3 in differentiated human SH-SY5Y cells Neuroscience181:226-33 2010 |
| PubMed ID: 21333717 |
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| Marasa BS, Srikantan S, Martindale JL, Kim MM, Lee EK, Gorospe M, Abdelmohsen K, MicroRNA profiling in human diploid fibroblasts uncovers miR-519 role in replicative senescence Aging2:333-43 2010 |
| PubMed ID: 20606251 |
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| Li B, Jog SP, Reddy S, Comai L, WRN controls formation of extrachromosomal telomeric circles and is required for TRF2DeltaB-mediated telomere shortening Molecular and cellular biology28:1892-904 2008 |
| PubMed ID: 18212065 |
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| Dauth I, Krüger J, Hofmann TG, Homeodomain-interacting protein kinase 2 is the ionizing radiation-activated p53 serine 46 kinase and is regulated by ATM Cancer research67:2274-9 2007 |
| PubMed ID: 17332358 |
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| Tresini M, Lorenzini A, Torres C, Cristofalo VJ, Modulation of replicative senescence of diploid human cells by nuclear ERK signaling J Biol Chem282(6):4136-51 2007 |
| PubMed ID: 17145763 |
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| Singh NK, Singh NN, Androphy EJ, Singh RN, Splicing of a critical exon of human Survival Motor Neuron is regulated by a unique silencer element located in the last intron Molecular and cellular biology26:1333-46 2006 |
| PubMed ID: 16449646 |
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| Gosslau A, Chen M, Ho CT, Chen KY, A methoxy derivative of resveratrol analogue selectively induced activation of the mitochondrial apoptotic pathway in transformed fibroblasts Br J Cancer92(3):513-21 2005 |
| PubMed ID: 15668717 |
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| Michishita E, Park JY, Burneskis JM, Barrett JC, Horikawa I, Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins Molecular biology of the cell16:4623-35 2005 |
| PubMed ID: 16079181 |
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| Wang W, Martindale JL, Yang X, Chrest FJ, Gorospe M., Increased stability of the p16 mRNA with replicative senescence. EMBO Rep6(2):158-64 2005 |
| PubMed ID: 15678155 |
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| Kahl CR, Means AR, Regulation of cyclin D1/Cdk4 complexes by calcium/calmodulin-dependent protein kinase I. J Biol Chem279(15):15411-9 2004 |
| PubMed ID: 14754892 |
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| Kahl CR, Means AR, Calcineurin regulates cyclin D1 accumulation in growth-stimulated fibroblasts. Mol Biol Cell15(4):1833-42 2004 |
| PubMed ID: 14767060 |
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| Marchesini N, Osta W, Bielawski J, Luberto C, Obeid LM, Hannun YA, Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells. J Biol Chem279(24):25101-11 2004 |
| PubMed ID: 15051724 |
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| Sedelnikova OA, Horikawa I, Zimonjic DB, Popescu NC, Bonner WM, Barrett JC, Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks. Nat Cell Biol6(2):168-70 2004 |
| PubMed ID: 14755273 |
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| Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T, Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev18(17):2108-19 2004 |
| PubMed ID: 15342490 |
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| Von Kobbe C, May A, Grandori C, Bohr VA, Werner syndrome cells escape hydrogen peroxide-induced cell proliferation arrest. FASEB J18(15):1970-2 2004 |
| PubMed ID: 15459124 |
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| Xin H, Pereira-Smith OM, Choubey D, Role of IFI 16 in cellular senescence of human fibroblasts. Oncogene23(37):6209-17 2004 |
| PubMed ID: 15208661 |
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| Baird DM, Rowson J, Wynford-Thomas D, Kipling D, Extensive allelic variation and ultrashort telomeres in senescent human cells. Nat Genet33(2):203-7 2003 |
| PubMed ID: 12539050 |
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| Li GZ, Eller MS, Firoozabadi R, Gilchrest BA, Evidence that exposure of the telomere 3' overhang sequence induces senescence Proceedings of the National Academy of Sciences of the United States of America100:527-31 2003 |
| PubMed ID: 12515865 |
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| Schawalder J, Paric E, Neff NF, Telomere and ribosomal DNA repeats are chromosomal targets of the bloom syndrome DNA helicase. BMC Cell Biol4:15:527-31 2003 |
| PubMed ID: 14577841 |
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| Ding W, Gao S, Scott RE, Senescence represses the nuclear localization of the serum response factor and differentiation regulates its nuclear localization with lineage specificity. J Cell Sci114(Pt 5):1011-8 2001 |
| PubMed ID: 11181183 |
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| Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, Lansdorp PM, Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet10(5):519-28 2001 |
| PubMed ID: 11181576 |
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| Lu J, Ho CT, Ghai G, Chen KY, Differential effects of theaflavin monogallates on cell growth, apoptosis, and Cox-2 gene expression in cancerous versus normal cells. Cancer Res60(22):6465-71 2000 |
| PubMed ID: 11103814 |
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| Luberto C, Hannun YA, Sphingomyelin synthase, a potential regulator of intracellular levels of ceramide and diacylglycerol during SV40 transformation. Does sphingomyelin synthase account for the putative phosphatidylcholine-specific phospholipase C? J Biol Chem273:14550-9 1998 |
| PubMed ID: 9603970 |
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| Gamard CJ, Dbaibo GS, Liu B, Obeid LM, Hannun YA, Selective involvement of ceramide in cytokine-induced apoptosis. Ceramide inhibits phorbol ester activation of nuclear factor kappaB. J Biol Chem272(26):16474-81 1997 |
| PubMed ID: 9195956 |
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| Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995 |
| PubMed ID: 7534307 |
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| Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, et al, A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A92:9363-7 1995 |
| PubMed ID: 7568133 |
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| Venable ME, Lee JY, Smyth MJ, Bielawska A, Obeid LM, Role of ceramide in cellular senescence. J Biol Chem270:30701-8 1995 |
| PubMed ID: 8530509 |
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| Dimri GP, Campisi J, Altered profile of transcription factor-binding activities in senescent human fibroblasts. Exp Cell Res212:132-40 1994 |
| PubMed ID: 8174635 |
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| Dimri GP, Hara E, Campisi J, Regulation of two E2F-related genes in presenescent and senescent human fibroblasts. J Biol Chem269:16180-6 1994 |
| PubMed ID: 8206919 |
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| Venable ME, Blobe GC, Obeid LM, Identification of a defect in the phospholipase D/diacylglycerol pathway in cellular senescence. J Biol Chem269:26040-4 1994 |
| PubMed ID: 7929315 |
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| Ganguly T, Duker NJ, Differential cell cycle modulation of human DNA glycosylases against oxidized pyrimidines. Mutat Res235:137-46 1990 |
| PubMed ID: 2308590 |
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| Kunisada T, Miller CD, Schneider EL, Ultraviolet light-induced DNA damage in transcribed sequences: no change in repair with age. Mutat Res237:75-81 1990 |
| PubMed ID: 2164149 |
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| Pereira-Smith OM, Stein GH, Robetorye S, Meyer-Demarest S, Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts. J Cell Physiol143:222-5 1990 |
| PubMed ID: 2332448 |
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| Gibson JM, Milam SB, Klebe RJ, Late passage cells display an increase in contractile behavior. Mech Ageing Dev48:101-10 1989 |
| PubMed ID: 2739467 |
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| Giordano T, Foster DN, Identification of a highly abundant cDNA isolated from senescent WI-38 cells. Exp Cell Res185:399-406 1989 |
| PubMed ID: 2480908 |
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| Giordano T, Kleinsek D, Foster DN, Increase in abundance of a transcript hybridizing to elongation factor I alpha during cellular senescence and quiescence. Exp Gerontol24:501-13 1989 |
| PubMed ID: 2483690 |
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| McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989 |
| PubMed ID: 2474570 |
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| Kondo H, Kasuga H, Noumura T, The heterogeneity of human fibroblasts as determined from the effects of hydrocortisone on cell growth and specific dexamethasone binding. Exp Cell Res158:342-8 1985 |
| PubMed ID: 4007059 |
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| Hayflick, The limited in vitro lifetime of human diploid cell strains. Exp Cell Res37:614 (1965):342-8 1965 |
| PubMed ID: 4007059 |
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| Hayflick, The serial cultivation of human diploid cell strains. Exp Cell Res25:585 (1961):342-8 1961 |
| PubMed ID: 4007059 |
| Cumulative PDL at Freeze |
34 |
| Passage Frozen |
26 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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