GM00010
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Unspecified
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
|
Race
|
Black/African American
|
|
Ethnicity
|
PUERTO RICAN
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Karyotypic analysis and Case history
|
|
ISCN
|
46,XY
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| LYSOSOMAL TRANSPORT PROCESSES |
Pisoni and Thoene (J Biol Chem 264:4850-4856,1989) investigated the manner in which nucleosides resulting from the lysosomal degredation of nucleic acids were released from lysosomes. Lysosomal adenosine uptake studies indicated that the uptake was saturable and specifically, competitively inhibited by other nucleosides. The authors concluded that this indicated the existence of a carrier-mediated transport system for nucleosides within fibroblast lysosomal membranes. |
| |
| Remarks |
46,XY; skin biopsy; 12 cm crown-rump length; same patient as GM01380A; 4% of cells show random chromosome loss and 2% show random chromosome aberrations |
| Ling JQ, Hou A, Hoffman AR, Long-range DNA interactions are specifically altered by locked nucleic acid-targeting of a CTCF binding site Biochimica et biophysica acta1809:24-33 2010 |
| PubMed ID: 21111075 |
| |
| Ling Y, Maile LA, Badley-Clarke J, Clemmons DR, DOK1 mediates SHP-2 binding to the alphaVbeta3 integrin and thereby regulates insulin-like growth factor I signaling in cultured vascular smooth muscle cells The Journal of biological chemistry280:3151-8 2004 |
| PubMed ID: 15546884 |
| |
| Park MA, Thoene JG, Potential role of apoptosis in development of the cystinotic phenotype Pediatric nephrology (Berlin, Germany)20:441-6 2004 |
| PubMed ID: 15622500 |
| |
| Moralez A, Busby WH Jr, Clemmons D, Control of insulin-like growth factor binding protein-5 protease synthesis and
secretion by human fibroblasts and porcine aortic smooth muscle cells. Endocrinology144(6):2489-95 2003 |
| PubMed ID: 12746311 |
| |
| Park M, Helip-Wooley A, Thoene J, Lysosomal cystine storage augments apoptosis in cultured human fibroblasts and
renal tubular epithelial cells. J Am Soc Nephrol13(12):2878-87 2002 |
| PubMed ID: 12444206 |
| |
| Imai Y, Moralez A, Andag U, Clarke JB, Busby WH Jr, Clemmons DR, Substitutions for hydrophobic amino acids in the N-terminal domains of IGFBP-3 and -5 markedly reduce IGF-I binding and alter their biologic actions. J Biol Chem275:18188-94 2000 |
| PubMed ID: 10766744 |
| |
| Thoene J, Lemons R, Anikster Y, Mullet J, Paelicke K, Lucero C, Gahl W, Schneider J, Shu SG, Campbell HT, Mutations of CTNS causing intermediate cystinosis. Mol Genet Metab67(4):283-93 1999 |
| PubMed ID: 10444339 |
| |
| Arai T, Clarke J, Parker A, Busby W Jr, Nam T, Clemmons DR, Substitution of specific amino acids in insulin-like growth factor (IGF) binding protein 5 alters heparin binding and its change in affinity for IGF-I response to heparin. J Biol Chem271:6099-106 1996 |
| PubMed ID: 8626396 |
| |
| Duan C, Hawes SB, Prevette T, Clemmons DR, Insulin-like growth factor-I (IGF-I) regulates IGF-binding protein-5 synthesis through transcriptional activation of the gene in aortic smooth muscle cells. J Biol Chem271:4280-8 1996 |
| PubMed ID: 8626775 |
| |
| Parker A, Clarke JB, Busby WH Jr, Clemmons DR, Identification of the extracellular matrix binding sites for insulin- like growth factor-binding protein 5. J Biol Chem271:13523-9 1996 |
| PubMed ID: 8662813 |
| |
| Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995 |
| PubMed ID: 7559606 |
| |
| Pisoni RL, Park GY, Velilla VQ, Thoene JG, Detection and characterization of a transport system mediating cysteamine entry into human fibroblast lysosomes. Specificity for aminoethylthiol and aminoethylsulfide derivatives. J Biol Chem270:1179-84 1995 |
| PubMed ID: 7836377 |
| |
| Wani G, Wani AA, D'Ambrosio SM, Cell type-specific expression of the O6-alkylguanine-DNA alkyltransferase gene in normal human liver tissues as revealed by in situ hybridization. Carcinogenesis14:737-41 1993 |
| PubMed ID: 8472340 |
| |
| Camacho-Hubner C, Busby WH Jr, McCusker RH, Wright G, Clemmons DR, Identification of the forms of insulin-like growth factor-binding proteins produced by human fibroblasts and the mechanisms that regulate their secretion. J Biol Chem267:11949-56 1992 |
| PubMed ID: 1376315 |
| |
| Mirzayans R, Middlestadt MV, Paterson MC, Cytotoxic and mutagenic effects of methylnitrosourea in two human fetal fibroblast strains differing in O6-methylguanine-DNA methyltransferase activity. Carcinogenesis13:1185-90 1992 |
| PubMed ID: 1638685 |
| |
| Lemons RM, Thoene JG, Mediated calcium transport by isolated human fibroblast lysosomes. J Biol Chem266:14378-82 1991 |
| PubMed ID: 1830585 |
| |
| McCusker RH, Camacho-Hubner C, Bayne ML, Cascieri MA, Clemmons DR, Insulin-like growth factor (IGF) binding to human fibroblast and glioblastoma cells: the modulating effect of cell released IGF binding proteins (IGFBPs). J Cell Physiol144:244-53 1990 |
| PubMed ID: 2166057 |
| |
| Pisoni RL, Thoene JG, Detection and characterization of a nucleoside transport system in human fibroblast lysosomes. J Biol Chem264:4850-6 1989 |
| PubMed ID: 2925670 |
| |
| Richter MR, Graves DT, DNA synthesis in U-2 OS human osteosarcoma cells is independent of PDGF binding to functional cell surface receptors. J Cell Physiol135:474-80 1988 |
| PubMed ID: 2840434 |
| |
| Pisoni RL, Flickinger KS, Thoene JG, Christensen HN, Characterization of carrier-mediated transport systems for small neutral amino acids in human fibroblast lysosomes. J Biol Chem262:6010-7 1987 |
| PubMed ID: 3106350 |
| |
| Clemmons DR, Elgin RG, Han VK, Casella SJ, D'Ercole AJ, Van Wyk JJ, Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. J Clin Invest77:1548-56 1986 |
| PubMed ID: 2422210 |
| |
| Dracopoli NC, Rettig WJ, Whitfield GK, Darlington GJ, Spengler BA, Biedler JL, Old LJ, Kourides IA, Assignment of the gene for the beta subunit of thyroid-stimulating hormone to the short arm of human chromosome 1. Proc Natl Acad Sci U S A83:1822-1826 1986 |
| PubMed ID: 3006075 |
| |
| Imada M, Imada S, A 200 kDa surface glycoprotein of human fibroblasts encoded by a gene on chromosome 11 is regulated by cyclic AMP. Biochim Biophys Acta885:162-9 1986 |
| PubMed ID: 3004600 |
| |
| Imada M, Kao FT, Law ML, Jones C, Identification of 200,000-dalton human cell surface protein encoded by gene mapped to long arm of chromosome 11. Somat Cell Mol Genet12:197-201 1986 |
| PubMed ID: 3457478 |
| |
| Asselineau D, Bernhard B, Bailly C, Darmon M, Epidermal morphogenesis and induction of the 67 kD keratin polypeptide by culture of human keratinocytes at the liquid-air interface. Exp Cell Res159:536-9 1985 |
| PubMed ID: 2411581 |
| |
| Chu SH, Hoban CJ, Owen AJ, Geyer RP, Platelet-derived growth factor stimulates rapid polyphosphoinositide breakdown in fetal human fibroblasts. J Cell Physiol124:391-6 1985 |
| PubMed ID: 2995418 |
| |
| Coates PM, Hale DE, Stanley CA, Corkey BE, Cortner JA, Genetic deficiency of medium-chain acyl coenzyme A dehydrogenase: studies in cultured skin fibroblasts and peripheral mononuclear leukocytes. Pediatr Res19:671-6 1985 |
| PubMed ID: 4022673 |
| |
| Dracopoli NC, Rettig WJ, Albino AP, Esposito D, Archidiacono N, Rocchi M, Siniscalco M, Old LJ, Genes controlling gp25/30 cell-surface molecules map to chromosomes X and Y and escape X-inactivation. Am J Hum Genet37:199-207 1985 |
| PubMed ID: 4038849 |
| |
| Hale DE, Batshaw ML, Coates PM, Frerman FE, Goodman SI, Singh I, Stanley CA, Long-chain acyl coenzyme A dehydrogenase deficiency: an inherited cause of nonketotic hypoglycemia. Pediatr Res19:666-71 1985 |
| PubMed ID: 4022672 |
| |
| Pisoni RL, Thoene JG, Christensen HN, Detection and characterization of carrier-mediated cationic amino acid transport in lysosomes of normal and cystinotic human fibroblasts. Role in therapeutic cystine removal? J Biol Chem260:4791-8 1985 |
| PubMed ID: 3921538 |
| |
| Betsholtz C, Westermark B, Growth factor-induced proliferation of human fibroblasts in serum-free culture depends on cell density and extracellular calcium concentration. J Cell Physiol118:203-10 1984 |
| PubMed ID: 6607257 |
| |
| Graves DT, Antoniades HN, Williams SR, Owen AJ, Evidence for functional platelet-derived growth factor receptors on MG- 63 human osteosarcoma cells. Cancer Res44:2966-70 1984 |
| PubMed ID: 6327031 |
| |
| Lawrence DA, Pircher R, Kryceve-Martinerie C, Jullien P, Normal embryo fibroblasts release transforming growth factors in a latent form. J Cell Physiol121:184-8 1984 |
| PubMed ID: 6090474 |
| |
| Clemmons DR, Age dependent production of a competence factor by human fibroblasts. J Cell Physiol114:61-7 1983 |
| PubMed ID: 6826661 |
| |
| Clemmons DR, Shaw DS, Variables controlling somatomedin production by cultured human fibroblasts. J Cell Physiol115:137-42 1983 |
| PubMed ID: 6601662 |
| |
| Graves DT, Owen AJ, Antoniades HN, Evidence that a human osteosarcoma cell line which secretes a mitogen similar to platelet-derived growth factor requires growth factors present in platelet-poor plasma. Cancer Res43:83-7 1983 |
| PubMed ID: 6336604 |
| |
| Lieberman MA, Mitogenic proteins of the 3T3 plasma membrane. J Cell Physiol114:73-6 1983 |
| PubMed ID: 6826663 |
| |
| Van Keuren, Proteins affected by chromosome 21 and ageing in vitro (from Gene Expression in Normal and Transformed Cells, Plenum Pub Corp; Celis & Bravo, Eds) "Gene Exp in Norm & Trans Cells"1983,pp349:73-6 1983 |
| PubMed ID: 6826663 |
| |
| Van Keuren ML, Merril CR, Goldman D, Protein variations associated with in vitro aging of human fibroblasts and quantitative limits on the error catastrophe hypothesis. J Gerontol38:645-52 1983 |
| PubMed ID: 6226730 |
| |
| Chen DJ, Okinaka RT, Strniste GF, Barnhart BJ, Induction of 6-thioguanine-resistant mutations by rat-liver homogenate (S9)-activated promutagens in human embryonic skin fibroblasts. Mutat Res101:87-98 1982 |
| PubMed ID: 7078562 |
| |
| Rosenthal MD, Whitehurst MC, Selective utilization of omega 6 and omega 3 polyunsaturated fatty acids by human skin fibroblasts. J Cell Physiol113:298-306 1982 |
| PubMed ID: 6294123 |
| |
| Starling JJ, Sieg SM, Beckett ML, Schellhammer PF, Ladaga LE, Wright GL Jr, Monoclonal antibodies to human prostate and bladder tumor-associated antigens. Cancer Res42:3084-9 1982 |
| PubMed ID: 7046915 |
| |
| Van Keuren ML, Goldman D, Merril CR, Protein variations associated with Down's syndrome, chromosome 21, and Alzheimer's disease. Ann N Y Acad Sci396:55-67 1982 |
| PubMed ID: 6217777 |
| |
| Rosenthal MD, Accumulation of neutral lipids by human skin fibroblasts: differential effects of saturated and unsaturated fatty acids. Lipids16:173-82 1981 |
| PubMed ID: 7219087 |
| |
| Scott, Stimulation of lipid synthesis in human skin fibroblasts by platelet derived growth factor. Fed Proc40:351 (1981):173-82 1981 |
| PubMed ID: 7219087 |
| |
| Van Keuren, A quantitative two-dimensional electrophoretic survey of proteins affected by chromosome 21 (from Electrophoresis '81, Walter de Gruyter & Co., Allen, Arnaud eds.) "Electrophoresis '81"1981, pp 355-369:173-82 1981 |
| PubMed ID: 7219087 |
| |
| Rosenthal, Promotion of triacylglycerol synthesis in human skin fibroblasts by saturated & unsaturated fatty acids. Fed Proc39:1724 (1980):173-82 1980 |
| PubMed ID: 7219087 |
| |
| Rosenthal MD, Selectivity in incorporation, utilization and retention of oleic and linoleic acids by human skin fibroblasts. Lipids15:838-48 1980 |
| PubMed ID: 7442473 |
| |
| Somers KD, Murphey MM, Cytochalasin B-induced multinucleation of human tumor and normal cell cultures. Cell Biol Int Rep4:487-95 1980 |
| PubMed ID: 7397790 |
| |
| Rosenthal, Differential utilization of oleate and linoleate by human skin fibroblasts. Fed Proc38:649 (1979):487-95 1979 |
| PubMed ID: 7397790 |
| |
| Rosenthal, Incorporation of moderate and excessive levels of fatty acids by human skin fibroblasts. Fed Proc37:1832 (1978):487-95 1978 |
| PubMed ID: 7397790 |
| |
| Rosenthal MD, Geyer RP, Release of phospholipid acyl groups from fetal human fibroblasts. Biochem Biophys Res Commun74:1456-62 1977 |
| PubMed ID: 843371 |
| |
| Slayback JR, Cheung LW, Geyer RP, Quantitative extraction of microgram amounts of lipid from cultured human cells. Anal Biochem83:372-84 1977 |
| PubMed ID: 603031 |
| |
| Slayback JR, Cheung LW, Geyer RP, Comparative effects of human platelet growth factor on the growth and morphology of human fetal and adult diploid fibroblasts. Exp Cell Res110:462-6 1977 |
| PubMed ID: 590364 |
| |
| Rosenthal MD, Geyer RP, Phospholipid acyl group stability in cultured fibroblasts. Differences between human cell lines of fetal and adult origin. Biochim Biophys Acta441:465-76 1976 |
| PubMed ID: 974094 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|