GM00011
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
46,XY; skin biopsy; 2% of cells show random chromosome loss |
| Bayerl J, Ayyash M, Shani T, Manor YS, Gafni O, Massarwa R, Kalma Y, Aguilera-Castrejon A, Zerbib M, Amir H, Sheban D, Geula S, Mor N, Weinberger L, Naveh Tassa S, Krupalnik V, Oldak B, Livnat N, Tarazi S, Tawil S, Wildschutz E, Ashouokhi S, Lasman L, Rotter V, Hanna S, Ben-Yosef D, Novershtern N, Viukov S, Hanna JH, Principles of signaling pathway modulation for enhancing human naive pluripotency induction Cell stem cell: 2020 |
| PubMed ID: 33915080 |
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| Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo GW, Winslow MM, Bassik MC, Attardi LD, Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program Molecular cell80:452-469.e9 2020 |
| PubMed ID: 33157015 |
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| Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
| PubMed ID: 23665875 |
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| Gray SJ, Gerhardt J, Doerfler W, Small LE, Fanning E, An origin of DNA replication in the promoter region of the human fragile X mental retardation (FMR1) gene Molecular and cellular biology27:426-37 2006 |
| PubMed ID: 17101793 |
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| Tolstonog GV, Mothes E, Shoeman RL, Traub P, Isolation of SDS-stable complexes of the intermediate filament protein vimentin with repetitive, mobile, nuclear matrix attachment region, and mitochondrial DNA sequence elements from cultured mouse and human fibroblasts. DNA Cell Biol20(9):531-54 2001 |
| PubMed ID: 11747605 |
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| Broker BJ, Chakrabarti R, Blynman T, Roesler J, Wang MB, Srivatsan ES, Comparison of growth factor expression in fetal and adult fibroblasts: a preliminary report. Arch Otolaryngol Head Neck Surg125:676-80 1999 |
| PubMed ID: 10367926 |
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| Seavey SE, Holubar M, Saucedo LJ, Perry ME, The E7 oncoprotein of human papillomavirus type 16 stabilizes p53 through a mechanism independent of p19(ARF). J Virol73:7590-8 1999 |
| PubMed ID: 10438849 |
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| Eleftheriou CS, Trakas N, Kokla A, Tzartos SJ, A group of three fibroblast secreted polypeptides suppressed by cellular ageing and interferon-gamma. Biochim Biophys Acta1180:304-12 1993 |
| PubMed ID: 7678507 |
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| Wani G, Wani AA, D'Ambrosio SM, Cell type-specific expression of the O6-alkylguanine-DNA alkyltransferase gene in normal human liver tissues as revealed by in situ hybridization. Carcinogenesis14:737-41 1993 |
| PubMed ID: 8472340 |
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| Mirzayans R, Middlestadt MV, Paterson MC, Cytotoxic and mutagenic effects of methylnitrosourea in two human fetal fibroblast strains differing in O6-methylguanine-DNA methyltransferase activity. Carcinogenesis13:1185-90 1992 |
| PubMed ID: 1638685 |
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| Maher VM, Domoradzki J, Bhattacharyya NP, Tsujimura T, Corner RC, McCormick JJ, Alkylation damage, DNA repair and mutagenesis in human cells. Mutat Res233:235-45 1990 |
| PubMed ID: 2233805 |
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| Sester U, Sawada M, Wang E, Purification and biochemical characterization of statin, a nonproliferation specific protein from rat liver. J Biol Chem265:19966-72 1990 |
| PubMed ID: 2246273 |
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| Sano H, Shiomi N, Imanishi K, Maie O, Shiomi T, DNA methylation in xeroderma pigmentosum. Mutat Res217:141-51 1989 |
| PubMed ID: 2918867 |
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| Ching G, Wang E, Absence of three secreted proteins and presence of a 57-kDa protein related to irreversible arrest of cell growth. Proc Natl Acad Sci U S A85:151-5 1988 |
| PubMed ID: 3422412 |
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| Connolly JA, Sarabia VE, Kelvin DJ, Wang E, The disappearance of a cyclin-like protein and the appearance of statin is correlated with the onset of differentiation during myogenesis in vitro. Exp Cell Res174:461-71 1988 |
| PubMed ID: 2892689 |
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| Wang E, Contact-inhibition-induced quiescent state is marked by intense nuclear expression of statin. J Cell Physiol133:151-7 1987 |
| PubMed ID: 3312241 |
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| Tani K, Takizawa T, Yoshida A, Normal mRNA content in a phosphoglycerate kinase variant with severe enzyme deficiency. Am J Hum Genet37:931-7 1985 |
| PubMed ID: 3840329 |
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| Ben-Ishai R, Sharon R, Rothman M, Miskin R, DNA repair and induction of plasminogen activator in human fetal cells treated with ultraviolet light. Carcinogenesis5:357-62 1984 |
| PubMed ID: 6538462 |
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| Domoradzki J, Pegg AE, Dolan ME, Maher VM, McCormick JJ, Correlation between O6-methylguanine-DNA-methyltransferase activity and resistance of human cells to the cytotoxic and mutagenic effect of N- methyl-N'-nitro-N-nitrosoguanidine. Carcinogenesis5:1641-7 1984 |
| PubMed ID: 6499116 |
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| Tamm I, Kikuchi T, Wang E, Pfeffer LM, Growth rate of control and beta-interferon-treated human fibroblast populations over the course of their in vitro life span. Cancer Res44:2291-6 1984 |
| PubMed ID: 6722770 |
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| Wang E, Gundersen D, Increased organization of cytoskeleton accompanying the aging of human fibroblasts in vitro. Exp Cell Res154:191-202 1984 |
| PubMed ID: 6540707 |
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| Meager A, Graves HE, Shuttleworth J, Zucker N, Interferon production: variation in yields from human cell lines. Infect Immun25:658-63 1979 |
| PubMed ID: 489124 |
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| Chen YT, Worthy TE, Krooth RS, Evidence for a striking increase in acetylcholinesterase activity in cultured human fibroblasts which are trisomic for chromosome two. Somatic Cell Genet4:265-98 1978 |
| PubMed ID: 694721 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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