GM00043
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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Black/African American
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Family Member
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2
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Relation to Proband
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mother
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.18 |
| Passage Frozen |
9 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy; 46,XX; 2% of cells show random loss of a single chromosome; daughter is GM00038B |
| Pepponi R, De Simone R, De Nuccio C, Visentin S, Matteucci A, Bernardo A, Popoli P, Ferrante A, Repurposing Dipyridamole in Niemann Pick Type C Disease: A Proof of Concept Study International journal of molecular sciences23: 2022 |
| PubMed ID: 35408815 |
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| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
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| Visentin S, De Nuccio C, Bernardo A, Pepponi R, Ferrante A, Minghetti L, Popoli P, The stimulation of adenosine A2A receptors ameliorates the pathological phenotype of fibroblasts from Niemann-Pick type C patients The Journal of neuroscience : the official journal of the Society for Neuroscience33:15388-93 2013 |
| PubMed ID: 24068806 |
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| Burrow JW, Koch JA, Chuang HH, Zhong W, Dean DD, Sylvia VL, Nitric oxide donors selectively reduce the expression of matrix metalloproteinases-8 and -9 by human diabetic skin fibroblasts The Journal of surgical research140:90-8 2006 |
| PubMed ID: 17418871 |
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| Wang G, Chuang L, Zhang X, Colton S, Dombkowski A, Reiners J, Diakiw A, Xu XS, The initiative role of XPC protein in cisplatin DNA damaging treatment-mediated cell cycle regulation. Nucleic Acids Res32(7):2231-40 2004 |
| PubMed ID: 15107491 |
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| Famulski KS, Al-Hijailan RS, Dobler K, Pienkowska M, Al-Mohanna F, Paterson MC, Aberrant sensing of extracellular Ca2+ by cultured ataxia telangiectasia fibroblasts. Oncogene22(3):471-5 2003 |
| PubMed ID: 12545170 |
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| Mirzayans R, Famulski KS, Enns L, Fraser M, Paterson MC, Characterization of the signal transduction pathway mediating gamma ray- induced inhibition of DNA synthesis in human cells: indirect evidence for involvement of calmodulin but not protein kinase C nor p53. Oncogene11:1597-605 1995 |
| PubMed ID: 7478584 |
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| Mirzayans R, Paterson MC, Dose-dependent increase in repair of
1-beta-D-arabinofuranosylcytosine-detectable DNA lesions in UV-treated xeroderma
pigmentosum (group A) fibroblasts. Mutat Res262(3):151-7 1991 |
| PubMed ID: 2002813 |
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| Mirzayans R, Smith BP, Paterson MC, Hypersensitivity to cell killing and faulty repair of 1-beta-D- arabinofuranosylcytosine-detectable sites in human (ataxia- telangiectasia) fibroblasts treated with 4-nitroquinoline 1-oxide. Cancer Res49:5523-9 1989 |
| PubMed ID: 2507129 |
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| Connelly PW, Ranganathan S, Maguire GF, Lee M, Myher JJ, Kottke BA, Kuksis A, Little JA, The beta very low density lipoprotein present in hepatic lipase deficiency competitively inhibits low density lipoprotein binding to fibroblasts and stimulates fibroblast acyl-CoA:cholesterol acyltransferase. J Biol Chem263:14184-8 1988 |
| PubMed ID: 3170542 |
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| Mirzayans R, Liuzzi M, Paterson MC, Methylmethanesulfonate-induced DNA damage and its repair in cultured human
fibroblasts: normal rates of induction and removal of alkali-labile sites in
xeroderma pigmentosum (group A) cells. Carcinogenesis9(12):2257-63 1988 |
| PubMed ID: 3191570 |
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| Ranganathan S, Matsuura H, Kottke BA, Studies on the effect of storage of normolipidemic plasma on the metabolism of very-low-density lipoprotein cholesterol in human skin fibroblasts. Biochim Biophys Acta960:125-9 1988 |
| PubMed ID: 3162811 |
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| Matsuura H, Ranganathan S, Yamamoto M, Kattke BA, Studies on the effect of concanavalin A on the metabolism of low density lipoproteins in skin fibroblasts. Jpn J Med26:323-5 1987 |
| PubMed ID: 3694915 |
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| Yamamoto M, Ranganathan S, Kottke BA, Metabolism of glycosylated very-low-density lipoproteins in human skin fibroblasts. Biochim Biophys Acta875:410-3 1986 |
| PubMed ID: 3942775 |
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| Mirzayans R, Paterson MC, Waters R, Defective repair of a class of 4NQO-induced alkali-labile DNA lesions in xeroderma pigmentosum complementation group A fibroblasts. Carcinogenesis6:555-9 1985 |
| PubMed ID: 3921271 |
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| Yamamoto M, Ranganathan S, Kottke BA, Interaction of normolipidemic very low density lipoproteins with receptors in human skin fibroblasts. Biochem Int11:573-81 1985 |
| PubMed ID: 3853463 |
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| Ranganathan S, Jackson RL, Effect of calcium-channel-blocking drugs on lysosomal function in human skin fibroblasts. Biochem Pharmacol33:2377-82 1984 |
| PubMed ID: 6466359 |
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| Ranganathan S, Harmony JA, Jackson RJ, Effect of Ca2+ blocking agents on the metabolism of low density lipoproteins in human skin fibroblasts. Biochem Biophys Res Commun107:217-24 1982 |
| PubMed ID: 7126204 |
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| Ranganathan S, Jackson RL, Harmony JA, Effect of pantethine on the biosynthesis of cholesterol in human skin fibroblasts. Atherosclerosis44:261-73 1982 |
| PubMed ID: 7150392 |
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| Bech-Hansen NT, Sell BM, Mulvihill JJ, Paterson MC, Association of in vitro radiosensitivity and cancer in a family with acute myelogenous leukemia. Cancer Res41:2046-50 1981 |
| PubMed ID: 6940653 |
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| Gray PN, May PC, Mundy L, Elkins J, L-Glutamate toxicity in Huntington's disease fibroblasts. Biochem Biophys Res Commun95:707-14 1980 |
| PubMed ID: 6448048 |
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| Krepinsky AB, Rainbow AJ, Heddle JA, Studies on the ultraviolet light sensitivity of Bloom's syndrome fibroblasts. Mutat Res69:357-68 1980 |
| PubMed ID: 7360151 |
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| Nambudiri AM, Ranganathan S, Rudney H, The role of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in the regulation of ubiquinone synthesis in human fibroblasts. J Biol Chem255:5894-9 1980 |
| PubMed ID: 7380842 |
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| Chen YT, Worthy TE, Krooth RS, Evidence for a striking increase in acetylcholinesterase activity in cultured human fibroblasts which are trisomic for chromosome two. Somatic Cell Genet4:265-98 1978 |
| PubMed ID: 694721 |
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| Yatscoff RW, Goldstein S, Freeman KB, Conservation of genes coding for proteins synthesized in human mitochondria. Somatic Cell Genet4:633-45 1978 |
| PubMed ID: 741350 |
| Passage Frozen |
9 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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