Description:
RING CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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45,XY,-9[28]/46,XY,r(9)[22]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
44% of cells have r(9); 56% are 45,XY, -9; trigonocephaly, hypertelorism, systolic murmur, and multiple facial malformations |
| Kistenmacher ML, Punnett HH, Aronson M, Miller RC, Greene AE, Coriell LL, A ring 9 chromosome. Repository identification no. GM-166. Cytogenet Cell Genet15:122-3 1975 |
| PubMed ID: 1183236 |
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| Kistenmacher ML, Punnett HH, Comparative behavior of ring chromosomes. Am J Hum Genet22:304-18 1970 |
| PubMed ID: 5445002 |
| Passage Frozen |
4 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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