GM00215
Fibroblast from Lung, Lung
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
| Remarks |
Line V79; young adult male; established by Ford and Yerganian; cloned by Elkind; fibroblast culture established from a lung biopsy; near diploid |
| Canonero R, Martelli A, Marinari UM, Brambilla G, Mutation induction in Chinese hamster lung V79 cells by five alk-2- enals produced by lipid peroxidation. Mutat Res244:153-6 1990 |
| PubMed ID: 2355937 |
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| Brambilla G, Robbiano L, Cajelli E, Martelli A, Turmolini F, Mazzei M, Cytotoxic, DNA-damaging and mutagenic properties of 2,6-dimethoxy-1,4- benzoquinone, formed by dimethophrine-nitrite interaction. J Pharmacol Exp Ther244:1011-5 1988 |
| PubMed ID: 3252018 |
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| Reiners JJ Jr, Colby AB, Survey of cytotoxicities and antimutagenic and antitumor initiating activities of Cu(II)(3,5-diisopropylsalicylate)2 and its analogs in a keratinocyte-mediated mutation assay and the murine skin multistage carcinogenesis model. Carcinogenesis9:629-32 1988 |
| PubMed ID: 3128407 |
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| Bonifas JM, Morley BJ, Oakey RE, Kan YW, Epstein EH Jr, Cloning of a cDNA for steroid sulfatase: frequent occurrence of gene deletions in patients with recessive X chromosome-linked ichthyosis. Proc Natl Acad Sci U S A84:9248-51 1987 |
| PubMed ID: 3480541 |
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| Cajelli E, Canonero R, Martelli A, Brambilla G, Methylglyoxal-induced mutation to 6-thioguanine resistance in V79 cells. Mutat Res190:47-50 1987 |
| PubMed ID: 3796671 |
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| Cajelli E, Ferraris A, Brambilla G, Mutagenicity of 4-hydroxynonenal in V79 Chinese hamster cells. Mutat Res190:169-71 1987 |
| PubMed ID: 3821775 |
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| Soloff BL, Nagle WA, Moss AJ Jr, Henle KJ, Crawford JT, Apoptosis induced by cold shock in vitro is dependent on cell growth phase. Biochem Biophys Res Commun145:876-83 1987 |
| PubMed ID: 3593376 |
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| Koval, Intrinsic resistance to the lethal effects of x-irradiation in insect and arachnid cells. Proc Natl Acad Sci USA80:4752 (1983):876-83 1983 |
| PubMed ID: 3593376 |
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| Lee JC, Bahr GF, Microfluorometric studies on chromosomes. Quantitative determination of protein content of Chinese hamster chromosome 1 in situ with and without trypsin digestion. Chromosoma88:374-6 1983 |
| PubMed ID: 6653206 |
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| Tetzner C, Juhl HJ, Rudiger HW, Sister-chromatid exchange induction by metabolically activated retinoids in human diploid fibroblast cultures. Mutat Res79:163-7 1980 |
| PubMed ID: 6933309 |
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| Goldstein JL, Helgeson JA, Brown MS, Inhibition of cholesterol synthesis with compactin renders growth of cultured cells dependent on the low density lipoprotein receptor. J Biol Chem254:5403-9 1979 |
| PubMed ID: 221469 |
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| Elkind, Radiation response of mammalian cells grown in culture. I. Repair of X-ray damage in surviving Chinese hamster cells. Radiat Res13:556 (1960):5403-9 1960 |
| PubMed ID: 221469 |
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| Ford, Observations on the chromosomes of Chinese hamster cells in tissue culture. J Natl Cancer Inst21:393 (1958):5403-9 1958 |
| PubMed ID: 221469 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
5% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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