GM00302
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.35 |
| Passage Frozen |
17 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy; formerly GM00075; normal B-hexosaminidase and B-galactosidase; |
| Marentette JO, Anderson CC, Prutton KM, Jennings EQ, Rauniyar AK, Galligan JJ, Roede JR, Trisomy 21 impairs PGE2 production in dermal fibroblasts Prostaglandins & other lipid mediators153:106524 2020 |
| PubMed ID: 33418267 |
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| Lee SW1, Oh YM2, Lu YL3, Kim WK1, Yoo AS4., MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts Developmental Cell46:73-84 2018 |
| PubMed ID: 29974865 |
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| Zhou X, Cole SW, Rao NP, Cheng Z, Li Y, McBride J, Wong DT, Identification of discrete chromosomal deletion by binary recursive partitioning of microarray differential expression data Journal of medical genetics42:416-9 2005 |
| PubMed ID: 15863671 |
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| Zhou X, Cole SW, Hu S, Wong DT, Detection of DNA copy number abnormality by microarray expression analysis Human genetics114:464-7 2004 |
| PubMed ID: 14872338 |
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| Zhou X, Mok SC, Chen Z, Li Y, Wong DT, Concurrent analysis of loss of heterozygosity (LOH) and copy number abnormality (CNA) for oral premalignancy progression using the Affymetrix 10K SNP mapping array Human genetics115:327-30 2004 |
| PubMed ID: 15290239 |
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| Kim BE, Smith K, Petris MJ, A copper treatable Menkes disease mutation associated with defective trafficking
of a functional Menkes copper ATPase. J Med Genet40(4):290-5 2003 |
| PubMed ID: 12676902 |
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| Weiss MJ, Ray K, Fallon MD, Whyte MP, Fedde KN, Lafferty MA, Mulivor RA, Harris H, Analysis of liver/bone/kidney alkaline phosphatase mRNA, DNA, and enzymatic activity in cultured skin fibroblasts from 14 unrelated patients with severe hypophosphatasia. Am J Hum Genet44:686-94 1989 |
| PubMed ID: 2705456 |
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| Lin PY, Gruenstein E, Identification of a defective cAMP-stimulated Cl- channel in cystic fibrosis fibroblasts. J Biol Chem262:15345-7 1987 |
| PubMed ID: 2445735 |
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| Guernsey DL, Koebbe M, Thomas JE, Myerly TK, Zmolek D, An altered response in the induction of cell membrane (Na + K)ATPase by thyroid hormone is characteristic of senescence in cultured human fibroblasts. Mech Ageing Dev33:283-93 1986 |
| PubMed ID: 3012221 |
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| Hinman LM, Ksiezak-Reding H, Baker AC, Blass JP, Pigeon liver phosphoprotein phosphatase: an effective activator of pyruvate dehydrogenase in tissue homogenates. Arch Biochem Biophys246:381-90 1986 |
| PubMed ID: 3008658 |
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| Sephel GC, Davidson JM, Elastin production in human skin fibroblast cultures and its decline with age. J Invest Dermatol86:279-85 1986 |
| PubMed ID: 3745952 |
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| Saito M, Rosenberg A, The fate of glucosylceramide (glucocerebroside) in genetically impaired (lysosomal beta-glucosidase deficient) Gaucher disease diploid human fibroblasts. J Biol Chem260:2295-300 1985 |
| PubMed ID: 3919000 |
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| Pato CN, Davis MH, Doughty MJ, Bryant SH, Gruenstein E, Increased membrane permeability to chloride in Duchenne muscular dystrophy fibroblasts and its relationship to muscle function. Proc Natl Acad Sci U S A80:4732-6 1983 |
| PubMed ID: 6576355 |
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| Davis MH, Pato CN, Grinvalsky H, Gruenstein E, Identification of a biochemical difference between male and female human fibroblasts: implications for the expression of Duchenne muscular dystrophy. Neurology32:951-7 1982 |
| PubMed ID: 7202167 |
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| Nichols WW, Bradt CI, Toji LH, Godley M, Segawa M, Induction of sister chromatid exchanges by transformation with simian virus 40. Cancer Res38:960-4 1978 |
| PubMed ID: 205353 |
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| Breslow JL, Epstein J, Vayo MM, Fontaine JH, Decreased ouabain binding in cystic fibrosis fibroblasts in potassium- free medium. Exp Cell Res110:399-407 1977 |
| PubMed ID: 590361 |
| Passage Frozen |
17 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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