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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
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| Genbank |
COX1 |
| Remarks |
Line A-9; mouse L cell; fibroblast-like; HPRT and APRT deficient; selectable with 8-azaguanine; heteroploid; passage 8 at CCR |
| Heikoop JC, Hogervorst FB, Meershoek EJ, Grootscholten PM, den Dunnen JT, van Ommen GJ, Expression of the human Dp 71 (apo-dystrophin-1) gene from a 760-kb DMD- YAC transferred to mouse cells. Eur J Hum Genet3:168-79 1995 |
| PubMed ID: 7583042 |
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| Yokoi H, Hadano S, Kogi M, Kang X, Wakasa K, Ikeda JE, Isolation of expressed sequences encoded by the human Xq terminal portion using microclone probes generated by laser microdissection. Genomics20:404-11 1994 |
| PubMed ID: 8034313 |
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| Featherstone T, Huxley C, Extrachromosomal maintenance and amplification of yeast artificial chromosome DNA in mouse cells. Genomics17:267-78 1993 |
| PubMed ID: 8406477 |
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| Gnirke A, Huxley C, Peterson K, Olson MV, Microinjection of intact 200- to 500-kb fragments of YAC DNA into mammalian cells. Genomics15:659-67 1993 |
| PubMed ID: 8385648 |
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| LaForgia S, Lasota J, Latif F, Boghosian-Sell L, Kastury K, Ohta M, Druck T, Atchison L, Cannizzaro LA, Barnea G, et al, Detailed genetic and physical map of the 3p chromosome region surrounding the familial renal cell carcinoma chromosome translocation, t(3;8)(p14.2;q24.1). Cancer Res53:3118-24 1993 |
| PubMed ID: 8319219 |
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| Salido EC, Passage MB, Yen PH, Shapiro LJ, Mohandas TK, An evaluation of the inactive mouse X chromosome in somatic cell hybrids. Somat Cell Mol Genet19:65-71 1993 |
| PubMed ID: 8460399 |
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| Troelstra C, Landsvater RM, Wiegant J, van der Ploeg M, Viel G, Buys CH, Hoeijmakers JH, Localization of the nucleotide excision repair gene ERCC6 to human chromosome 10q11-q21. Genomics12:745-9 1992 |
| PubMed ID: 1349298 |
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| Bols NC, Mosser DD, Boliska SA, Transfer of purine metabolites between cells through the medium and via cell contacts in cocultures of HGPRT+ and HGPRT- cells. Exp Cell Res173:206-17 1987 |
| PubMed ID: 3678380 |
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| Kucera R, Paulus H, Localization of the deoxyribonucleotide biosynthetic enzymes ribonucleotide reductase and thymidylate synthase in mouse L cells. Exp Cell Res167:417-28 1986 |
| PubMed ID: 3533576 |
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| Yen PH, Mohandas T, Shapiro LJ, Stability of DNA methylation of the human hypoxanthine phosphoribosyltransferase gene. Somat Cell Mol Genet12:153-61 1986 |
| PubMed ID: 3008350 |
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| Fedde K, Horwitz AL, Complementation of multiple sulfatase deficiency in somatic cell hybrids. Am J Hum Genet36:623-33 1984 |
| PubMed ID: 6731437 |
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| Carlin CR, Identification of cell surface polypeptides encoded by human chromosomes 2 and 5 in human-mouse somatic cell hybrids. Cytogenet Cell Genet35:226-32 1983 |
| PubMed ID: 6861528 |
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| Boliska SA, Bols NC, The transfer of purine metabolites via the medium rather than through cell contacts. Exp Cell Res141:303-12 1982 |
| PubMed ID: 7140841 |
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| Carlin CR, Rao KW, Sequential staining with Hoechst 33258 and quinacrine mustard for the identification of human chromosomes in somatic cell hybrids. Exp Cell Res138:466-9 1982 |
| PubMed ID: 6176460 |
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| Mohandas T, Sparkes RS, Shapiro LJ, Genetic evidence for the inactivation of a human autosomal locus attached to an inactive X chromosome. Am J Hum Genet34:811-7 1982 |
| PubMed ID: 7124733 |
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| Sparkes, Regional mapping of the human PGM1 locus. Cytogenet Cell Genet32:319 (1982):811-7 1982 |
| PubMed ID: 7124733 |
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| Koeffler HP, Sparkes RS, Stang H, Mohandas T, Regional assignment of genes for human alpha-globin and phosphoglycollate phosphatase to the short arm of chromosome 16. Proc Natl Acad Sci U S A78:7015-8 1981 |
| PubMed ID: 6273902 |
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| Clynes MM, Smyth H, O'Kennedy R, Corrigan A, Separation of viable from nonviable cells using a discontinuous density gradient. In Vitro16:809-12 1980 |
| PubMed ID: 7409839 |
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| Mohandas T, Sparkes RS, Hellkuhl B, Grzeschik KH, Shapiro LJ, Expression of an X-linked gene from an inactive human X chromosome in mouse-human hybrid cells: further evidence for the noninactivation of the steroid sulfatase locus in man. Proc Natl Acad Sci U S A77:6759-63 1980 |
| PubMed ID: 6935682 |
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| Mohandas T, Shapiro LJ, Sparkes RS, Sparkes MC, Regional assignment of the steroid sulfatase-X-linked ichthyosis locus: implications for a noninactivated region on the short arm of human X chromosome. Proc Natl Acad Sci U S A76:5779-83 1979 |
| PubMed ID: 293682 |
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| Sundar Raj CV, Church RL, Klobutcher LA, Ruddle FH, Genetics of the connective tissue proteins: assignment of the gene for human type I procollagen to chromosome 17 by analysis of cell hybrids and microcell hybrids. Proc Natl Acad Sci U S A74:4444-8 1977 |
| PubMed ID: 412188 |
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| Cos RP, Drauss MR, Balis ME, Dancis J, Letter: Mouse fibroblasts A9 are deficient in HPRT and APRT. Am J Hum Genet26:272-3 1974 |
| PubMed ID: 4823035 |
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| Tischfield JA, Ruddle FH, Assignment of the gene for adenine phosphoribosyltransferase to human chromosome 16 by mouse-human somatic cell hybridization. Proc Natl Acad Sci U S A71:45-9 1974 |
| PubMed ID: 4129802 |
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| Littlefield JW, The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exp Cell Res41:190-6 1966 |
| PubMed ID: 4952025 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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