Description:
TRANSLOCATED CHROMOSOME
LEUKEMIA, SUB-ACUTE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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45,XX,der(13;22)(13qter>13q10::22q10>22qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
9 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome 13: TRANSLOCATION Breakpoint 13cen t(13;22)13cen |
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Chromosome 22: TRANSLOCATION Breakpoint 22cen t(13;22)22cen |
| Remarks |
Myelomonocytic leukemia |
| Page SL, Shin JC, Han JY, Choo KH, Shaffer LG, Breakpoint diversity illustrates distinct mechanisms for Robertsonian translocation formation. Hum Mol Genet5(9):1279-88 1996 |
| PubMed ID: 8872467 |
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| Alfi O, Miller RC, Greene AE, Coriell LL, A (13;22) Robertsonian translocation, 45 chromosomes. Repository identification no. GM-392. Cytogenet Cell Genet15:55-6 1975 |
| PubMed ID: 1058758 |
| Passage Frozen |
9 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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