GM00458
Fibroblast from Skin, Unspecified
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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22,Y
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Line CHW; male; fibroblast cell line established by spontaneous transformation of a normal skin culture; pseudodiploid; 30% pseudotetraploid; 22,Y |
| Ono K, Kaku T, Makita N, Kitabatake A, Arita M, Selective block of late currents in the DeltaKPQ Na(+) channel mutant by pilsicainide and lidocaine with distinct mechanisms. Mol Pharmacol57(2):392-400 2000 |
| PubMed ID: 10648650 |
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| Kaneko K, Kobayashi H, Onodera O, Miyatake T, Tsuji S, Genomic organization of a cDNA (QM) demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells and its localization to Xq28. Hum Mol Genet1:529-33 1992 |
| PubMed ID: 1339145 |
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| Fedde K, Horwitz AL, Complementation of multiple sulfatase deficiency in somatic cell hybrids. Am J Hum Genet36:623-33 1984 |
| PubMed ID: 6731437 |
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| Gee PA, Mohandas RT, Douglas GR, Palser HR, Richardson BJ, Hamerton JL, Characteristics of an HPRT-deficient chinese hamster cell line. Cytogenet Cell Genet13:437-47 1974 |
| PubMed ID: 4462979 |
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| Lin CC, Chang TD, Niewczas-Late V, The establishment and chromosome analysis of a new cell line of Chinese hamster from spontaneous transformation in vitro. Can J Genet Cytol13:9-13 1971 |
| PubMed ID: 5563334 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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