Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
46,XY; 14.5 cm crown-rump length; skin biopsy; 4% of cells show random chromosome loss |
| Broker BJ, Chakrabarti R, Blynman T, Roesler J, Wang MB, Srivatsan ES, Comparison of growth factor expression in fetal and adult fibroblasts: a preliminary report. Arch Otolaryngol Head Neck Surg125:676-80 1999 |
| PubMed ID: 10367926 |
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| Litt MD, Hansen RS, Hornstra IK, Gartler SM, Yang TP, 5-Azadeoxycytidine-induced chromatin remodeling of the inactive X-linked HPRT gene promoter occurs prior to transcription factor binding and gene reactivation. J Biol Chem272:14921-6 1997 |
| PubMed ID: 9169463 |
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| Hornstra IK, Yang TP, High-resolution methylation analysis of the human hypoxanthine phosphoribosyltransferase gene 5' region on the active and inactive X chromosomes: correlation with binding sites for transcription factors. Mol Cell Biol14:1419-30 1994 |
| PubMed ID: 8289817 |
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| David M, Grimley PM, Finbloom DS, Larner AC, A nuclear tyrosine phosphatase downregulates interferon-induced gene expression. Mol Cell Biol13:7515-21 1993 |
| PubMed ID: 8246969 |
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| Hornstra IK, Yang TP, Multiple in vivo footprints are specific to the active allele of the X-linked human hypoxanthine phosphoribosyltransferase gene 5' region: implications for X chromosome inactivation. Mol Cell Biol12:5345-54 1992 |
| PubMed ID: 1448069 |
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| Petricoin EF 3d, Hackett RH, Akai H, Igarashi K, Finbloom DS, Larner AC, Modulation of interferon signaling in human fibroblasts by phorbol esters. Mol Cell Biol12:4486-95 1992 |
| PubMed ID: 1406637 |
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| Pienta KJ, Getzenberg RH, Coffey DS, Characterization of nuclear morphology and nuclear matrices in ageing human fibroblasts. Mech Ageing Dev62:13-24 1992 |
| PubMed ID: 1560681 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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