Description:
TRANSLOCATED CHROMOSOME
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Chromosome Abnormalities |
|
Cell Type
|
Fibroblast
|
|
Transformant
|
Untransformed
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
46,XX,t(4;15)(4pter>4q11::15p11>15pter; 15qter>15p11::4q11>4qter)
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Cytogenetics |
Chromosome 15: TRANSLOCATION Breakpoint 15p11 t(4;15)15p11 |
|
Chromosome 4: TRANSLOCATION Breakpoint 4q11 t(4;15)4q11 |
| Remarks |
22% of cells show an additional t(6;11)(q13;q23) subline |
| Cohen MM, Ornoy A, Rosenmann A, Kohn G, An inherited translocation t(4;15) (p16;q22) leading to two cases of partial trisomy 15. Ann Genet18:99-103 1975 |
| PubMed ID: 1081372 |
| |
| Kohn G, Cohen MM, Aronson M, Miller RC, Greene AE, Coriell LL, A (4;15) translocation, balanced, 46 chromosomes. Repository identification no. GM-624. Cytogenet Cell Genet15:124-5 1975 |
| PubMed ID: 1183237 |
| |
| Kohn G, Ornoy A, Cohen MM, Prenatal diagnosis: a problem in verification. J Med Genet11:247-8 1974 |
| PubMed ID: 4431030 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|