GM00730
Fibroblast from Skin, Arm
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.09 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| DNA LIGASE I AND II |
Lehmann et al (Cancer Res 48:6343-6347,1988) reported that this cell culture showed normal joining of Okazaki fragments during DNA replication. Willis et al (Carcinogenesis 10:217-219,1989) reported normal DNA ligase I activity with normal heat stability in extracts of this fibroblast culture. |
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| Remarks |
Skin biopsy (forearm); formerly GM00238; 46,XX/92,XXXX; 42%/58% |
| Ilieva M, Miller HE, Agarwal A, Paulus GK, Madsen JH, Bishop AJR, Kauppinen S, Uchida S, FibroDB: Expression Analysis of Protein-Coding and Long Non-Coding RNA Genes in Fibrosis Non-coding RNA8: 2022 |
| PubMed ID: 35202087 |
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| Charazac A, Fayyad N, Beal D, Bourgoin-Voillard S, Seve M, Sauvaigo S, Lamartine J, Soularue P, Moratille S, Martin MT, Ravanat JL, Douki T, Rachidi W, Impairment of Base Excision Repair in Dermal Fibroblasts Isolated From Nevoid Basal Cell Carcinoma Patients Frontiers in oncology10:1551 2020 |
| PubMed ID: 32850458 |
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| Mirzayans R, Famulski KS, Enns L, Fraser M, Paterson MC, Characterization of the signal transduction pathway mediating gamma ray- induced inhibition of DNA synthesis in human cells: indirect evidence for involvement of calmodulin but not protein kinase C nor p53. Oncogene11:1597-605 1995 |
| PubMed ID: 7478584 |
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| Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994 |
| PubMed ID: 8106562 |
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| Nagasawa H, Little JB, Tsang NM, Saunders E, Tesmer J, Strniste GF, Effect of dose rate on the survival of irradiated human skin fibroblasts. Radiat Res132:375-9 1992 |
| PubMed ID: 1475361 |
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| Stephenson C, Brivet M, Gautier M, Deschatrette J, Gitzelmann R, Karran P, Normal expression of thymidine kinase and O6-methylguanine-DNA methyltransferase in cultured fibroblasts from individuals with hereditary galactokinase deficiency. Biochem Genet29:135-44 1991 |
| PubMed ID: 1859354 |
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| Little JB, Nichols WW, Troilo P, Nagasawa H, Strong LC, Radiation sensitivity of cell strains from families with genetic disorders predisposing to radiation-induced cancer. Cancer Res49:4705-14 1989 |
| PubMed ID: 2758405 |
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| Mirzayans R, Smith BP, Paterson MC, Hypersensitivity to cell killing and faulty repair of 1-beta-D- arabinofuranosylcytosine-detectable sites in human (ataxia- telangiectasia) fibroblasts treated with 4-nitroquinoline 1-oxide. Cancer Res49:5523-9 1989 |
| PubMed ID: 2507129 |
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| Willis AE, Spurr NK, Lindahl T, Concomitant reversion of the characteristic phenotypic properties of a cell line of Bloom's syndrome origin. Carcinogenesis10:217-9 1989 |
| PubMed ID: 2910526 |
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| Lehmann AR, Willis AE, Broughton BC, James MR, Steingrimsdottir H, Harcourt SA, Arlett CF, Lindahl T, Relation between the human fibroblast strain 46BR and cell lines representative of Bloom's syndrome. Cancer Res48:6343-7 1988 |
| PubMed ID: 3180052 |
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| Nagasawa H, Burke MJ, Little FF, McCone EF, Chan GL, Little JB, Multiple abnormalities in the ultraviolet light response of cultured fibroblasts derived from patients with the basal cell nevus syndrome. Teratog Carcinog Mutagen8:25-33 1988 |
| PubMed ID: 2897722 |
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| Nagasawa H, Little JB, Radiosensitivities of ten apparently normal human diploid fibroblast strains to cell killing, G2-phase chromosomal aberrations, and cell cycle delay. Cancer Res48:4535-8 1988 |
| PubMed ID: 3396006 |
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| Nagasawa H, Kraemer KH, Shiloh Y, Little JB, Detection of ataxia telangiectasia heterozygous cell lines by postirradiation cumulative labeling index: measurements with coded samples. Cancer Res47:398-402 1987 |
| PubMed ID: 3791230 |
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| Tyrrell RM, Amaudruz F, Evidence for two independent pathways of biologically effective excision repair from its rate and extent in cells cultured from sun-sensitive humans. Cancer Res47:3725-8 1987 |
| PubMed ID: 3109732 |
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| Nove J, Little JB, Mayer PJ, Troilo P, Nichols WW, Hypersensitivity of cells from a new chromosomal-breakage syndrome to DNA-damaging agents. Mutat Res163:255-62 1986 |
| PubMed ID: 3785261 |
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| Tyrrell RM, Pidoux M, Quantitative differences in host cell reactivation of ultraviolet- damaged virus in human skin fibroblasts and epidermal keratinocytes cultured from the same foreskin biopsy. Cancer Res46:2665-9 1986 |
| PubMed ID: 2421882 |
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| Henderson LM, Arlett CF, Harcourt SA, Lehmann AR, Broughton BC, Cells from an immunodeficient patient (46BR) with a defect in DNA ligation are hypomutable but hypersensitive to the induction of sister chromatid exchanges. Proc Natl Acad Sci U S A82:2044-8 1985 |
| PubMed ID: 3856882 |
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| Nagasawa H, Latt SA, Lalande ME, Little JB, Effects of X-irradiation on cell-cycle progression, induction of chromosomal aberrations and cell killing in ataxia telangiectasia (AT) fibroblasts. Mutat Res148:71-82 1985 |
| PubMed ID: 3969079 |
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| Poirier V, James MR, Arlett CF, Lehmann AR, NAD and the synthesis of (ADP-ribose)n in a human cell strain (46BR) hypersensitive to the lethal effects of 3-aminobenzamide. Carcinogenesis6:837-41 1985 |
| PubMed ID: 2988809 |
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| Schwenn MR, Weichselbaum RR, Little JB, Investigation of the cytotoxic effects of DNA damaging agents on neurofibromatosis cells. Mutat Res142:55-8 1985 |
| PubMed ID: 3919286 |
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| Weichselbaum RR, Tomkinson K, Little JB, Repair of potentially lethal X-ray damage in fibroblasts derived from patients with hereditary and D-deletion retinoblastoma. Int J Radiat Biol Relat Stud Phys Chem Med47:445-56 1985 |
| PubMed ID: 3872857 |
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| Zamansky GB, Minka DF, Deal CL, Hendricks K, The in vitro photosensitivity of systemic lupus erythematosus skin fibroblasts. J Immunol134:1571-6 1985 |
| PubMed ID: 3968426 |
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| King AC, Monensin, like methylamine, prevents degradation of 125I-epidermal growth factor, causes intracellular accumulation of receptors and blocks the mitogenic response. Biochem Biophys Res Commun124:585-91 1984 |
| PubMed ID: 6093806 |
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| Mitchel RE, Chan A, Smith BP, Child SD, Paterson MC, The effects of hyperthermia and ionizing radiation in normal and ataxia telangiectasia human fibroblast lines. Radiat Res99(3):627-35 1984 |
| PubMed ID: 6473716 |
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| Arlett CF, Priestley A, Defective recovery from potentially lethal damage in some human fibroblast cell strains. Int J Radiat Biol43:157-167 1983 |
| PubMed ID: 6600729 |
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| Chan GL, Little JB, Cultured diploid fibroblasts from patients with the nevoid basal cell carcinoma syndrome are hypersensitive to killing by ionizing radiation. Am J Pathol111:50-5 1983 |
| PubMed ID: 6837723 |
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| Chua CC, Geiman DE, Ladda RL, Detection of an Mr 200,000 glycoprotein in the culture medium of skin fibroblasts from patients with Huntington disease. Biochem Biophys Res Commun111:690-9 1983 |
| PubMed ID: 6220707 |
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| Li FP, Little JB, Bech-Hansen NT, Paterson MC, Arlett C, Garnick MB, Mayer RJ, Acute leukemia after radiotherapy in a patient with Turcot's syndrome. Impaired colony formation in skin fibroblast cultures after irradiation. Am J Med74:343-8 1983 |
| PubMed ID: 6572034 |
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| Nagasawa H, Little JB, Suppression of cytotoxic effect of mitomycin-C by superoxide dismutase in Fanconi's anemia and dyskeratosis congenita fibroblasts. Carcinogenesis4:795-9 1983 |
| PubMed ID: 6409437 |
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| Nagasawa H, Little JB, Comparison of kinetics of X-ray-induced cell killing in normal, ataxia telangiectasia and hereditary retinoblastoma fibroblasts. Mutat Res109:297-308 1983 |
| PubMed ID: 6843573 |
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| Teo IA, Arlett CF, Harcourt SA, Priestley A, Broughton BC, Multiple hypersensitivity to mutagens in a cell strain (46BR) derived from a patient with immuno-deficiencies. Mutat Res107:371-86 1983 |
| PubMed ID: 6408472 |
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| Teo IA, Broughton BC, Day RS, James MR, Karran P, Mayne LV, Lehmann AR, A biochemical defect in the repair of alkylated DNA in cells from an immunodeficient patient (46BR). Carcinogenesis4:559-64 1983 |
| PubMed ID: 6850987 |
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| Mayne LV, Lehmann AR, Failure of RNA synthesis to recover after UV irradiation: an early defect in cells from individuals with Cockayne's syndrome and xeroderma pigmentosum. Cancer Res42:1473-8 1982 |
| PubMed ID: 6174225 |
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| Teo IA, Arlett CF, The response of a variety of human fibroblast cell strains to the lethal effects of alkylating agents. Carcinogenesis3:33-7 1982 |
| PubMed ID: 7067035 |
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| Pool, Rate measurements of retirement from the cell cycle by human fibroblasts in vitro. J Cell Biol91:11a (1981):33-7 1981 |
| PubMed ID: 7067035 |
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| Pool TB, Heitman TO, Buck MA, Changes in nuclear shape and mitochondrial structure do not accompany the loss of division potential in human fibroblasts in vitro. Am J Anat162:369-82 1981 |
| PubMed ID: 7325128 |
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| Brown, Two-dimensional analysis of radiolabeled proteins in cultured Huntington's Disease fibroblasts. Adv Neurol23:361 (1979):369-82 1979 |
| PubMed ID: 7325128 |
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| Torrelio BM, Paz MA, Increased phosphoribosylpyrophosphate synthetase activity in fibroblasts of hypoxanthine-guanine phosphoribosyl transferase deficient patients. Biochem Biophys Res Commun87:380-7 1979 |
| PubMed ID: 220971 |
| Passage Frozen |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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