GM00731
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
3.73 |
| Passage Frozen |
10 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
46,XY; skin biopsy |
| Zhu B, Fisher E, Li L, Zhong P, Yan Z, Feng J, PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes Stem cell reports18:2268-2282 2022 |
| PubMed ID: 37832540 |
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| Quist E, Trovato F, Avaliani N, Zetterdahl OG, Gonzalez-Ramos A, Hansen MG, Kokaia M, Canals I, Ahlenius H, Transcription factor-based direct conversion of human fibroblasts to functional astrocytes Stem cell reports17:1620-1635 2021 |
| PubMed ID: 35750047 |
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| Li H, Jiang H, Yin X, Bard JE, Zhang B, Feng J, Attenuation of PRRX2 and HEY2 enables efficient conversion of adult human skin fibroblasts to neurons Biochemical and biophysical research communications17:1620-1635 2019 |
| PubMed ID: 31255287 |
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| Song B, Cha Y, Ko S, Jeon J, Lee N, Seo H, Park KJ, Lee IH, Lopes C, Feitosa M, Luna MJ, Jung JH, Kim J, Hwang D, Cohen B, Teicher M, Leblanc P, Carter B, Kordower JH, Bolshakov VY, Kong SW, Schweitzer JS, Kim KS, Human Autologous iPSC-Derived Dopaminergic Progenitors Restore Motor Function in Parkinson's Disease Models The Journal of clinical investigation17:1620-1635 2019 |
| PubMed ID: 31714896 |
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| Cornacchia D, Zhang C, Zimmer B, Chung SY, Fan Y, Soliman MA, Tchieu J, Chambers SM, Shah H, Paull D, Konrad C, Vincendeau M, Noggle SA, Manfredi G, Finley LWS, Cross JR, Betel D, Studer L, Lipid Deprivation Induces a Stable, Naive-to-Primed Intermediate State of Pluripotency in Human PSCs Cell stem cell25:120-136.e10 2017 |
| PubMed ID: 31155483 |
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| Sassi-Gaha S, Loughlin DT, Kappler F, Schwartz ML, Su B, Tobia AM, Artlett CM, Two dicarbonyl compounds, 3-deoxyglucosone and methylglyoxal, differentially modulate dermal fibroblasts Matrix biology : journal of the International Society for Matrix Biology29:127-34 2009 |
| PubMed ID: 19800404 |
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| Greco M, Villani G, Mazzucchelli F, Bresolin N, Papa S, Attardi G, Marked aging-related decline in efficiency of oxidative phosphorylation in human skin fibroblasts The FASEB journal : official publication of the Federation of American Societies for Experimental Biology17:1706-8 2003 |
| PubMed ID: 12958183 |
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| Zhang J, Asin-Cayuela J, Fish J, Michikawa Y, Bonafe M, Olivieri F, Passarino G, De Benedictis G, Franceschi C, Attardi G, Strikingly higher frequency in centenarians and twins of mtDNA mutation causing remodeling of replication origin in leukocytes. Proc Natl Acad Sci U S A100(3):1116-21 2003 |
| PubMed ID: 12538859 |
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| Wang Y, Michikawa Y, Mallidis C, Bai Y, Woodhouse L, Yarasheski KE, Miller CA, Askanas V, Engel WK, Bhasin S, Attardi G, Muscle-specific mutations accumulate with aging in critical human mtDNA control sites for replication. Proc Natl Acad Sci U S A98(7):4022-7 2001 |
| PubMed ID: 11274426 |
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| Laderman KA, Penny JR, Mazzucchelli F, Bresolin N, Scarlato G, Attardi G, Aging-dependent functional alterations of mitochondrial DNA (mtDNA) from human fibroblasts transferred into mtDNA-less cells. J Biol Chem271(27):15891-7 1996 |
| PubMed ID: 8663253 |
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| Pienta KJ, Getzenberg RH, Coffey DS, Characterization of nuclear morphology and nuclear matrices in ageing human fibroblasts. Mech Ageing Dev62:13-24 1992 |
| PubMed ID: 1560681 |
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| Clemmons DR, Elgin RG, Han VK, Casella SJ, D'Ercole AJ, Van Wyk JJ, Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. J Clin Invest77:1548-56 1986 |
| PubMed ID: 2422210 |
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| Guernsey DL, Koebbe M, Thomas JE, Myerly TK, Zmolek D, An altered response in the induction of cell membrane (Na + K)ATPase by thyroid hormone is characteristic of senescence in cultured human fibroblasts. Mech Ageing Dev33:283-93 1986 |
| PubMed ID: 3012221 |
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| Conover CA, Dollar LA, Rosenfeld RG, Hintz RL, Somatomedin C-binding and action in fibroblasts from aged and progeric subjects. J Clin Endocrinol Metab60:685-91 1985 |
| PubMed ID: 2579088 |
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| Parshad R, Sanford KK, Jones GM, Chromosomal radiosensitivity during the G2 cell-cycle period of skin fibroblasts from individuals with familial cancer. Proc Natl Acad Sci U S A82:5400-3 1985 |
| PubMed ID: 3860870 |
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| Lieberman MA, Mitogenic proteins of the 3T3 plasma membrane. J Cell Physiol114:73-6 1983 |
| PubMed ID: 6826663 |
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| Hall JD, Almy RE, Scherer KL, DNA repair in cultured human fibroblasts does not decline with donor age. Exp Cell Res139:351-9 1982 |
| PubMed ID: 6282605 |
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| Pool, Rate measurements of retirement from the cell cycle by human fibroblasts in vitro. J Cell Biol91:11a (1981):351-9 1981 |
| PubMed ID: 6282605 |
| Passage Frozen |
10 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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