Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,t(2;8)(2pter>2q37::8q13>8qter;8pter>8q13::2q37>2qter)mat
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
10 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome 2: TRANSLOCATION Breakpoint 2q37 t(2;8)2q37 |
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Chromosome 2: TRANSLOCATION Breakpoint 2q37 |
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Chromosome 8: TRANSLOCATION Breakpoint 8q13 t(2;8)8q13 |
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Chromosome 8: TRANSLOCATION Breakpoint 8q13 |
| Remarks |
Secondary amenorrhea subsequently resolved spontaneously; mother has no history of amenorrhea |
| Yamanashi Y, Fukushige S, Semba K, Sukegawa J, Miyajima N, Matsubara K, Yamamoto T, Toyoshima K, The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol Cell Biol7:237-43 1987 |
| PubMed ID: 3561390 |
| |
| Bucknall WE, Aronson MM, Greene AE, Coriell LL, A (2;8) translocation, balanced, 46 chromosomes. Repository identification no. GM-845. Cytogenet Cell Genet21:111 1978 |
| PubMed ID: 648194 |
| Passage Frozen |
10 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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