GM00979
Erythroleukemic cell line
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Erythroleukemic cell line
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Transformant
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Friend Leukemia Virus
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6 Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Murine erythroleukemic line, T3-C12, derived from a splenic focus in DDD Mouse infected with Friend Leukemia virus; suspension culture; inducible for erythroid differentiation by DMSO; aneuploid; hypotetraploid |
| Brennan J, Mager D, Jefferies W, Takei F, Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J Exp Med180:2287-95 1994 |
| PubMed ID: 7964501 |
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| Cox WI, Specter S, Friedman H, Susceptibility of Friend erythroleukemia cells to natural cytotoxicity after in vitro treatment with dimethyl sulfoxide. Proc Soc Exp Biol Med169:337-42 1982 |
| PubMed ID: 7063517 |
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| Kaizu T, Turco SJ, Rush JS, Laine RA, Synthesis of the branched form of erythroglycan by Friend GM979 erythroleukemic cells. J Biol Chem257:8272-6 1982 |
| PubMed ID: 7085669 |
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| Pessano S, McNab A, Rovera G, Growth and differentiation of human and murine erythroleukemia cell lines in serum-free synthetic medium. Cancer Res41:3592-6 1981 |
| PubMed ID: 7020933 |
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| Seyedin SM, Pehrson JR, Cole RD, Loss of chromosomal high mobility group proteins HMG1 and HMG2 when mouse neuroblastoma and Friend erythroleukemia cells become committed to differentiation. Proc Natl Acad Sci U S A78:5988-92 1981 |
| PubMed ID: 6458811 |
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| Wu NC, Zucker RM, Mouse fetal hemoglobin synthesis in murine erythroleukemic cells. FEBS Lett99:299-302 1979 |
| PubMed ID: 284927 |
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| Dabney BJ, Beaudet AL, Increase in globin chains and globin mRNA in erythroleukemia cells in response to hemin. Arch Biochem Biophys179:106-12 1977 |
| PubMed ID: 265145 |
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| Orkin SH, Harosi FI, Leder P, Differentiation in erythroleukemic cells and their somatic hybrids. Proc Natl Acad Sci U S A72:98-102 1975 |
| PubMed ID: 164031 |
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| Ross J, Gielen J, Packman S, Ikawa Y, Leder P, Globin gene expression in cultured erythroleukemic cells. J Mol Biol87:697-714 1974 |
| PubMed ID: 4530120 |
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| Ross J, Ikawa Y, Leder P, Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. Proc Natl Acad Sci U S A69:3620-3 1972 |
| PubMed ID: 4509323 |
| Split Ratio |
1:25 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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