Description:
TERATOMA CELL LINE
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Cell Type
|
Fibroblast
|
Transformant
|
Untransformed
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XX
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remarks |
Passage 4 at IMR;PGM1=1;PGM3=1;PGD=AB; chromosome polymorphisms: 3=+/-;13=+/-; 15=+/-;21=+/-; uterine tissue; 8% of cells show random chromosome loss; same patient as GM01304 |
Linder D, McCaw BK, Hecht F, Parthenogenic origin of benign ovarian teratomas. N Engl J Med292:63-6 1975 |
PubMed ID: 162806 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|