GM01381
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
46,XY; skin biopsy; 10.5 cm crown-rump length; same patient as GM01379 |
| Suess PM, Smith SA, Morrissey JH, Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation Journal of thrombosis and haemostasis : JTH: 2020 |
| PubMed ID: 32808449 |
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| Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994 |
| PubMed ID: 8106562 |
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| Morrissey JH, Fakhrai H, Edgington TS, Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell50:129-35 1987 |
| PubMed ID: 3297348 |
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| Wilhelm, Human fibroblast collagenase: Glycosylat ion and tissue-specific levels of enzyme synthesis. Proc Natl Acad Sci USA83:3756 (1986):129-35 1986 |
| PubMed ID: 3297348 |
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| Vitkauskas G, Canellakis ES, Quantitation of metabolic cooperation by measurement of HGPRTase activity. Exp Cell Res156:537-43 1985 |
| PubMed ID: 2578405 |
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| Ben-Ishai R, Sharon R, Rothman M, Miskin R, DNA repair and induction of plasminogen activator in human fetal cells treated with ultraviolet light. Carcinogenesis5:357-62 1984 |
| PubMed ID: 6538462 |
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| Jaffe EA, Ruggiero JT, Leung LK, Doyle MJ, McKeown-Longo PJ, Mosher DF, Cultured human fibroblasts synthesize and secrete thrombospondin and incorporate it into extracellular matrix. Proc Natl Acad Sci U S A80:998-1002 1983 |
| PubMed ID: 6341993 |
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| Van Keuren, Proteins affected by chromosome 21 and ageing in vitro (from Gene Expression in Normal and Transformed Cells, Plenum Pub Corp; Celis & Bravo, Eds) "Gene Exp in Norm & Trans Cells"1983,pp349:998-1002 1983 |
| PubMed ID: 6341993 |
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| Nove J, Nichols WW, Weichselbaum RR, Little JB, Abnormalities of human chromosome 13 and in vitro radiosensitivity; a study of 19 fibroblast strains. Mutat Res84:157-67 1981 |
| PubMed ID: 7329430 |
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| Weichselbaum RR, Nove J, Albert D, Little JB, An in vitro investigation of genetic susceptibility to cancer in diploid fibroblasts from retinoblastoma patients. Teratog Carcinog Mutagen1:171-9 1980 |
| PubMed ID: 6119809 |
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| Weichselbaum RR, Nove J, Little JB, X-ray sensitivity of fifty-three human diploid fibroblast cell strains from patients with characterized genetic disorders. Cancer Res40:920-5 1980 |
| PubMed ID: 7471105 |
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| Little, Biological consequences of X-ray induced DNA damage and repair processes in relation to cell killing and carcinogenesis (from DNA Repair Mechanisms, Academic Press, Inc) "DNA Repair Mechanisms"1978, pp 701:920-5 1978 |
| PubMed ID: 7471105 |
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| Weichselbaum RR, Nove J, Little JB, X-ray sensitivity of diploid fibroblasts from patients with hereditary or sporadic retinoblastoma. Proc Natl Acad Sci U S A75:3962-4 1978 |
| PubMed ID: 279012 |
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| Weichselbaum RR, Nove J, Little JB, Skin fibroblasts from a D-deletion type retinoblastoma patient are abnormally X-ray sensitive. Nature266:726-7 1977 |
| PubMed ID: 876352 |
| Passage Frozen |
6 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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